The role of cyclic AMP in the regulation of exocytosis in the rat parotid gland: evidence obtained with the isoproterenol analog PI-39.

T N Spearman, J P Durham, F R Butcher
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Abstract

The isoproterenol analog, PI-39, induced a dose-dependent release of alpha-amylase from rat parotid minces in vitro. This effect was blocked by propranolol, a beta-adrenergic antagonist. PI-39 alone had no effect on parotid cyclic AMP levels or on protein kinase activation as assessed by the activity ratios method. However, PI-39 produced a dose-dependent increase in these parameters when a phosphodiesterase inhibitor was added to tissue minces simultaneously with the isoproterenol analog. Both isoproterenol and PI-39 altered the phosphorylation state of at least three specific endogenous phosphoproteins in (32P)-Pi prelabelled minces. The presence of a phosphodiesterase inhibitor was not required to demonstrate the effects of PI-39 on protein phosphorylation. Studies of endogenous protein phosphorylation in parotid broken cell preparations demonstrated that the phosphorylation of at least two of the PI-39 and isoproterenol-affected phosphoproteins are influenced by cyclic AMP. This study demonstrates that, under certain conditions, it is possible to activate a cyclic AMP-regulated biological process without elevating the total cellular cyclic AMP concentration or the protein kinase activity ratio.

环AMP在大鼠腮腺胞吐调节中的作用:异丙肾上腺素类似物PI-39获得的证据。
异丙肾上腺素类似物PI-39在体外诱导大鼠腮腺碎中α -淀粉酶的剂量依赖性释放。这种作用被一种-肾上腺素能拮抗剂心得安阻断了。通过活性比法评估,单独使用PI-39对腮腺环AMP水平或蛋白激酶激活没有影响。然而,当将磷酸二酯酶抑制剂与异丙肾上腺素类似物同时添加到组织肉末时,PI-39产生了这些参数的剂量依赖性增加。异丙肾上腺素和PI-39都改变了(32P)-Pi预标记肉末中至少三种特定内源性磷酸化蛋白的磷酸化状态。磷酸二酯酶抑制剂的存在并不需要证明PI-39对蛋白质磷酸化的影响。对腮腺破裂细胞制剂中内源性蛋白磷酸化的研究表明,至少有两种受PI-39和异丙肾上腺素影响的磷酸化蛋白受到环AMP的影响。本研究表明,在一定条件下,可以激活环AMP调节的生物过程,而不提高细胞总环AMP浓度或蛋白激酶活性比。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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