Cytofluorometric and cytophotometric measurements by using the exposure control device in the automatic photomicroscope.

Abstract

A simple method for quantitative measurements of staining and fluorescence intensities in microscopic preparation is described. The procedure is based on the use of the automatic exposure capability of the Zeiss Photomicroscope, in which the measuring value on the scale of the exposure control device after an arbitrary time permits the assessment of the light intensity on the point photosensor. The method has been checked through the study of chromatin staining and fluorescence by dyes and fluorochromes which are utilized in nucleic acids cytochemistry. The correpondence between these measurements and those obtained by using other methods is briefly discussed.