Effect of beta adrenergic agonists and beta blocking agents on hemopoiesis in human bone marrow.

Biomedicine / [publiee pour l'A.A.I.C.I.G.] Pub Date : 1981-09-01

C Dresch, J Minc, O Poirier, D Bouvet

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Abstract

The effect of propranolol (non specific blocking agent), acebutolol (beta 1 blocking agent), butoxamine (beta 2 blocking agent) and several beta adrenergic agonists was studied on 3H-thymidine (3H-TdR) incorporation and granulo-monocyte colony formation in agar by human bone marrow. Only butoxamine and propranolol decreased 3H-TdR incorporation by total normal bone marrow cells at concentrations above 10(-6) M for butoxamine and 10(-5) M for propranolol. Autoradiography showed that inhibition of 3H-TdR incorporation by butoxamine was slightly more pronounced on neutrophil precursors than on red cell precursors (neutrophil series LI..53 and erythroblasts .67 compared to control bone marrow cells at 10(-5) M concentration). The development of granulo-monocyte colonies in agar culture was delayed by preincubation with butoxamine at concentrations above 5 X 10(-6) M. Hydroxyurea suicide showed that this was due to a decrease in the number of CFU-C in S phase. beta 2 blocking agents are able to decrease the number of normal hematopoietic cells entering S phase. This effect is seen on both neutrophil and erythroblastic precursors and on granulo-monocyte progenitors. It could be used as a means of protecting bone marrow cells during cancer intensive chemotherapy.

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肾上腺素能激动剂和阻滞剂对人骨髓造血的影响。

研究了普萘洛尔(非特异性阻滞剂)、乙酰丁醇(β 1阻滞剂)、丁氧胺(β 2阻滞剂)和几种β肾上腺素能激动剂对人骨髓中3h -胸腺嘧啶(3H-TdR)的掺入和粒细胞单核细胞集落形成的影响。只有布toxamine和心得安在浓度大于10(-6)M和10(-5)M时，能减少正常骨髓细胞中3H-TdR的掺入。放射自显影显示，butoxamine对中性粒细胞前体的3H-TdR掺入的抑制作用略高于对红细胞前体(中性粒细胞系列LI)的抑制作用。在10(-5)M浓度下，与对照骨髓细胞相比，红母细胞为0.67。在琼脂培养中，浓度超过5 × 10(-6) m的butoxamine预孵育延迟了颗粒单核细胞菌落的发育。羟基脲自杀表明，这是由于S期CFU-C数量减少所致。β 2阻断剂能够减少正常造血细胞进入S期的数量。这种作用在中性粒细胞和红母细胞前体以及粒细胞单核细胞前体上都可见。它可以作为在癌症强化化疗期间保护骨髓细胞的一种手段。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedicine / [publiee pour l'A.A.I.C.I.G.]

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