{"title":"A new fluorimetric assay for streptothricins.","authors":"G Hesse, W Römer, N Miosga","doi":"10.1002/jobm.3630241010","DOIUrl":null,"url":null,"abstract":"<p><p>In strongly acidic medium (70% HClO4) streptothricins form a fluorophore (lambda ex = 312 nm; lambda em = 381 nm) with unknown structure. A fluorimetric determination of pure or crude products and cultures, respectively, was worked out based on this reaction. Concentrations for fluorescence measurements were in the range of 10(-8) - 2 X 10(-7) moles. Interferences of the assay are discussed, a statistical evaluation of results and a comparison between microbiological and fluorimetric findings are given.</p>","PeriodicalId":23874,"journal":{"name":"Zeitschrift fur allgemeine Mikrobiologie","volume":"24 10","pages":"709-11"},"PeriodicalIF":0.0000,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zeitschrift fur allgemeine Mikrobiologie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/jobm.3630241010","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
In strongly acidic medium (70% HClO4) streptothricins form a fluorophore (lambda ex = 312 nm; lambda em = 381 nm) with unknown structure. A fluorimetric determination of pure or crude products and cultures, respectively, was worked out based on this reaction. Concentrations for fluorescence measurements were in the range of 10(-8) - 2 X 10(-7) moles. Interferences of the assay are discussed, a statistical evaluation of results and a comparison between microbiological and fluorimetric findings are given.
在强酸性介质(70% HClO4)中，链霉素形成荧光团(λ ex = 312 nm;λ em = 381 nm)，结构未知。根据这一反应，分别对纯产物或粗产物和培养物进行了荧光测定。荧光测量的浓度范围为10(-8)- 2 X 10(-7)摩尔。讨论了测定的干扰，对结果进行了统计评价，并对微生物学和荧光学结果进行了比较。
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