Mechanism of ammonia production from aspartate in teleost liver

Comparative biochemistry and physiology Pub Date : 1970-11-01 DOI:10.1016/0010-406X(70)90962-X

Ralph Janicki , Jo Lingis

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引用次数: 11

Abstract

1.
1. Ammonia production from l-aspartate was studied in liver homogenates from four species of teleosts: Myoxocephalus scorpius, M octodecimspinosus, Pseudopleuronectes americanus and Anguilla rostrata. Liver homogenates from two species of elasmobranchs, Squalus acanthias and Raja erinacea, failed to liberate ammonia from l-aspartate.
2.
2. No ammonia was liberated from l-aspartate in liver homogenates from M. scorpius in the presence of transaminase inhibitors: 4 × 10⁻³ M semicarbizide, 5 × 10⁻³ M isonicotinoyl hydrazide and 4 × 10⁻³ M hydroxylamine.
3.
3. Single subcellular fractions, nuclear, mitochondrial and cytoplasmic, failed to produce ammonia from l-aspartate. However, when mitochondrial and cytoplasmic fractions were combined, ammonia was produced from l-aspartate.
4.
4. The results indicated a transamination reaction is involved in the liberation of ammonia from l-aspartate in teleost liver.

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硬骨鱼肝脏中天冬氨酸产氨的机制

1.1. 研究了四种硬骨鱼(Myoxocephalus scorpius, M octodecimspinosus, Pseudopleuronectes americanus和Anguilla rostrata)肝脏匀浆中l-天冬氨酸产氨的情况。棘角角鲨(Squalus acanthias)和角鼠(Raja erinacea)的肝脏匀浆不能从l-天冬氨酸中释放出氨。在转氨酶抑制剂:4 × 10−3 M半卡比脲、5 × 10−3 M异烟碱酰肼和4 × 10−3 M羟胺存在的情况下，天蝎蝎肝脏匀浆中的l-天冬氨酸没有释放氨。单个亚细胞组分，核，线粒体和细胞质，不能从l-天冬氨酸产生氨。然而，当线粒体和细胞质组分结合时，l-天冬氨酸产生氨。结果表明，在硬骨鱼肝脏中，氨从l-天冬氨酸中释放涉及转氨化反应。

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Comparative biochemistry and physiology

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