DNA synthesis in the developing ovary of the housefly

Comparative biochemistry and physiology Pub Date : 1970-10-01 DOI:10.1016/0010-406X(70)91031-5

Steve Miller, Jeanette M. Collins

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引用次数: 9

Abstract

1.
1. A method is described for the isolation of DNA from housefly ovaries where small quantities of the nucleic acid are present.
2.
2. Contamination of DNA by residual protein was reduced by treatment with proase. All the DNA samples were essentially free of RNA, as indicated by the orcinol reaction.
3.
3. The average molar base composition (40 per cent of guanine+cytosine) corresponds to Drosophila DNA, and the purine to pyrimidine ratio is close to unity, as would be expected for double stranded DNA.
4.
4. The conversion of (2-¹⁴C) glycine to purines of DNA was used as a measure of the de novo biosynthetic route. Analysis of the DNA, after hydrolysis, demonstrated that the label is present in the purine and pyrimidine bases.
5.
5. The specific activity is highest in the bases of DNA extracted from ovaries from 24-hr-old flies. The decrease in specific activity thereafter is due to the diluting effects in the de novo synthesis.

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家蝇卵巢发育中的DNA合成

1.1. 描述了一种从家蝇卵巢中分离DNA的方法，其中存在少量的核酸。用蛋白酶处理可减少残留蛋白对DNA的污染。从orcinol反应可以看出，所有的DNA样品基本上都不含RNA。平均摩尔碱基组成(鸟嘌呤+胞嘧啶的40%)与果蝇DNA相对应，嘌呤与嘧啶的比例接近统一，正如双链DNA所预期的那样。将(2-14C)甘氨酸转化为DNA的嘌呤作为从头生物合成途径的衡量标准。水解后的DNA分析表明，该标签存在于嘌呤和嘧啶碱基中。从24小时龄果蝇卵巢中提取的DNA碱基的比活性最高。之后比活性的降低是由于重新合成过程中的稀释作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Comparative biochemistry and physiology

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