Mapping plant lipids by paper chromatography

V.H. Booth
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引用次数: 4

Abstract

Two-dimensional paper chromatography has been adapted to the study of fat-soluble materials, which are thereby displayed mapwise on one sheet. The procedure, applied to a variety of plant tissues, has led to the discovery of reducing substances, pigments (including a new chlorophyll), fluorescing substances, quinones and unidentified phyllolipids; has helped in the differentiation of viruses; has uncovered a new enzyme or a new activity of an old one; has amplified knowledge on 20 substances that accompany chlorophyll and that may form part of the photosynthesizing apparatus; has aided the study of carotenoids from flowers, and indicated how mapping may be used in studying the origin of hybrids; has shown that mono-esters of carotenoids occur naturally, and given a hint that they may be common; and has played a major part in investigating the distribution, fluctuation and biochemical role of tocopherols in plants.

纸色谱法测定植物脂质
二维纸色谱法已适应于脂溶性材料的研究,从而在一张纸上显示地图。该方法应用于多种植物组织,发现了还原性物质、色素(包括一种新的叶绿素)、荧光物质、醌类和未识别的叶磷脂;有助于病毒的分化;发现了一种新的酶或一种旧酶的新活性;扩大了对20种伴随叶绿素的物质的认识,这些物质可能构成光合作用装置的一部分;曾帮助研究花中的类胡萝卜素,并指出如何利用绘图技术研究杂交植物的起源;表明类胡萝卜素单酯是自然产生的,并暗示它们可能很常见;在研究植物中生育酚的分布、波动和生化作用方面发挥了重要作用。
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