Some properties of proline-sRNA synthetase from rat liver

Clark Bublitz
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引用次数: 9

Abstract

  • 1.

    1. Some of the properties of a proline-sRNA synthetase from rat liver have been studied by either the hydroxamate assay or the proline-dependent 32PPi-ATP exchange.

  • 2.

    2. The enzyme was noticeably stabilized by sucrose. Although all preparations were stimulated by mercaptoethanol, some aged preparations had an absolute requirement for added mercaptan for activity.

  • 3.

    3. The enzyme required either magnesium, manganese, or calcium for activity. Hydroxamate formation was inhibited by PPi.

  • 4.

    4. [14C]Proline hydroxamate formation was inhibited by 3,4-dehydroproline; azetidine carboxylic acid; thiazolidine carboxylic acid; 2-methyl-, 2-ethyl-, or 2-propylthiazolidine carboxylic acid; thiazolidine; and mercaptoethylamine. Thiazolidine and mercaptoethylamine were competitive inhibitors of proline.

  • 5.

    5. The specific activity of extracts from younger rats was higher than that from older rats. Starvation also increased the activity of the extracts.

大鼠肝脏脯氨酸- srna合成酶的一些性质
1.1. 从大鼠肝脏提取的脯氨酸- srna合成酶的一些特性已经通过羟酸测定或脯氨酸依赖的32PPi-ATP交换进行了研究。蔗糖明显地稳定了这种酶。虽然所有的制剂都受到巯基乙醇的刺激,但有些陈化制剂的活性绝对需要添加巯基乙醇。这种酶需要镁、锰或钙才能发挥活性。PPi.4.4抑制羟酸酯的形成。[14C] 3,4-脱氢脯氨酸抑制脯氨酸羟酸酯的形成;氮杂丁羧酸;噻唑烷羧酸;2-甲基、2-乙基或2-丙基噻唑烷羧酸;thiazolidine;和mercaptoethylamine。噻唑烷和巯基乙胺是脯氨酸的竞争性抑制剂。年轻大鼠提取物的比活性高于年老大鼠提取物。饥饿也增加了提取物的活性。
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