Continuous gamma-irradiation of rats: dose-rate effect on loss and recovery of spermatogenesis.

Strahlentherapie Pub Date : 1985-07-01
G Pinon-Lataillade, J Maas
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Abstract

Male Sprague Dawley rats were continuously irradiated at a dose-rate of either 5 or 7 cGy/day, up to a total dose of 900 cGy. Changes in spermatogenesis with irradiation and the recovery of the testis during 33 weeks after irradiation were studied. No clear dose-rate effect with testicular weight occurred. During the irradiation time, increased dose and dose-rate induced a decrease in A spermatogonia and preleptotene spermatocyte number. In our experimental conditions germ cell production did not plateau, as shown by the increasing number of tubular cross sections devoid of germ cells beyond 500 cGy. The recovery of seminiferous epithelium occurred essentially within nine weeks. It was not dose-rate dependent and was still incomplete after 33 weeks. This lack of recovery might be due to limited compensatory division ability of the stem cells. Clusters of Sertoli cells were observed in the lumen of the seminiferous tubules; impaired function of these cells could also prevent the complete recovery of the seminiferous epithelium. By 16 weeks after the end of irradiation 67% of 5 cGy/day irradiated rats and 34% of 7 cGy/day irradiated rats recovered fertility.

大鼠连续γ辐照:对精子发生丧失和恢复的剂量率效应。
雄性Sprague Dawley大鼠以5或7 cGy/天的剂量率连续照射,直至总剂量为900 cGy。观察照射后精子发生的变化及照射后33周睾丸的恢复情况。睾丸重量没有明显的剂量率效应。随着辐照时间的延长,剂量和剂量率的增加导致a精原细胞和preleptotene精母细胞数量减少。在我们的实验条件下,生殖细胞的产生并没有趋于平稳,这表明在超过500 cGy时,缺乏生殖细胞的管状截面数量不断增加。精原上皮基本在9周内恢复。它不依赖于剂量率,并且在33周后仍然不完整。这种缺乏恢复可能是由于干细胞的代偿性分裂能力有限。精小管腔内可见成团的支持细胞;这些细胞的功能受损也会阻碍精原上皮的完全恢复。照射结束16周后,67%的5 cGy/天照射大鼠和34%的7 cGy/天照射大鼠恢复了生育能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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