Secreted phospholipases of the dimorphic fungus, Candida albicans; separation of three enzymes and some biological properties.

Y Banno, T Yamada, Y Nozawa
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引用次数: 89

Abstract

Several phospholipases are secreted into the culture medium by growing yeast cells of Candida albicans 3125. DEAE-Sephadex column chromatography of concentrated culture filtrate revealed three separable fractions with phospholipase activities. Analysis of products of hydrolysis showed that the enzyme activities were lysophospholipase, lysophospholipase-transacylase and a phospholipase B.

二态真菌白色念珠菌分泌磷脂酶;三种酶的分离及一些生物学特性。
几种磷脂酶是由白色念珠菌3125的酵母细胞分泌到培养基中。浓缩培养滤液DEAE-Sephadex柱层析显示三个可分离的磷脂酶活性组分。水解产物分析表明,酶活性为溶血磷脂酶、溶血磷脂酶转酰化酶和a磷脂酶B。
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