Ultrastructure of protoplasts from mycelium and microconidia of Trichophyton mentagrophytes.

E M Scott, S P Gorman, L R Wright
{"title":"Ultrastructure of protoplasts from mycelium and microconidia of Trichophyton mentagrophytes.","authors":"E M Scott,&nbsp;S P Gorman,&nbsp;L R Wright","doi":"10.1080/00362178585380061","DOIUrl":null,"url":null,"abstract":"<p><p>Protoplast formation from mycelium and microconidia of Trichophyton mentagrophytes was achieved with Novozym 234. Pretreatment procedures with dithiothreitol or urea mercaptoethanol sodium lauryl sulphate before digestion with Novozym 234 greatly reduced protoplast yield from mycelium. Snail gut enzyme did not protoplasts in good yield. Scanning electron microscopy of mycelium protoplasts showed the acquired spherical shape. The plasma membrane appeared finely granular although remnants of cell wall could sometimes be observed. Transmission electron microscopy showed the cell interior of these protoplasts was plasmolysed. Microconidia treated with Novozym 234 displayed a range of cell wall digestion, with intact protoplasts showing distinct cytoplasmic organelles.</p>","PeriodicalId":21469,"journal":{"name":"Sabouraudia","volume":"23 1","pages":"31-6"},"PeriodicalIF":0.0000,"publicationDate":"1985-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/00362178585380061","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sabouraudia","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/00362178585380061","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4

Abstract

Protoplast formation from mycelium and microconidia of Trichophyton mentagrophytes was achieved with Novozym 234. Pretreatment procedures with dithiothreitol or urea mercaptoethanol sodium lauryl sulphate before digestion with Novozym 234 greatly reduced protoplast yield from mycelium. Snail gut enzyme did not protoplasts in good yield. Scanning electron microscopy of mycelium protoplasts showed the acquired spherical shape. The plasma membrane appeared finely granular although remnants of cell wall could sometimes be observed. Transmission electron microscopy showed the cell interior of these protoplasts was plasmolysed. Microconidia treated with Novozym 234 displayed a range of cell wall digestion, with intact protoplasts showing distinct cytoplasmic organelles.

毛藓菌丝和微分生孢子原生质体的超微结构。
用Novozym 234可使毛癣菌的菌丝和微分生孢子形成原生质体。在用Novozym 234消化前,用二硫苏糖醇或尿素巯基乙醇十二烷基硫酸钠进行预处理,大大降低了菌丝体的原生质体产量。蜗牛肠酶没有高产的原生质体。菌丝原生质体的扫描电镜显示获得的球形。质膜呈细颗粒状,有时可见细胞壁残余。透射电镜显示,这些原生质体的细胞内部被质化。用Novozym 234处理的微分生孢子显示出一系列细胞壁消化,完整的原生质体显示出不同的细胞质细胞器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信