Hippocampal alterations in rats of early-stage hyperuricemia using diffusion kurtosis imaging: A preliminary study

IF 2.1 4区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Magnetic resonance imaging Pub Date : 2027-01-01 Epub Date: 2026-10-18 DOI:10.1016/j.mri.2026.110821

Ya-Lin Wang, Zhe Sun, Jin-Lu Song, Min-Jie Liang, You-Zhen Feng, Zhong-Yuan Cheng, Xiang-Ran Cai

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引用次数: 0

Abstract

Background

Although several quantitative methods are available for evaluating hyperuricemia-associated brain dysfunction, diffusion kurtosis imaging (DKI) exhibits greater sensitivity to subtle microstructural tissue changes, potentially enabling earlier detection of damage.

Purpose

To investigate the feasibility of hyperuricemia on cognitive impairment in rats using DKI.

Materials and methods

Fifty male rats were randomly divided into the normal control (CON) group and hyperuricemia model (HUA) group. Ten rats from each group were randomly selected and underwent longitudinal DKI scanning at baseline (week 0) and at weeks 2, 4, 6, and 8. DKI indicators were acquired with selecting bilateral symmetric regions of interest (ROI) in the hippocampal region, including, mean diffusivity (MD), fractional anisotropy (FA) and mean kurtosis (MK). The HE staining, GFAP and Iba-1 immunofluorescence staining were employed to reveal hippocampal histopathological changes. Additionally, the Morris water maze (MWM) test was applied to assess cognitive ability of rats at week 8th.

Result

In this study, the MD and FA values of hippocampal in HUA group exhibited a gradually decreasing trend, while the MK value gradually increased. Longitudinal analysis revealed earlier MK differences (weeks 4th–8th) and later FA divergences (weeks 6th–8th) between HUA and CON groups (p < 0.05). During weeks 4th–8th, the relative fluorescence intensity (RFI) of GFAP and, Iba-1 exhibited an inverse trend with FA value, whereas their changing trend was similar to that of MK value. Compared with controls, HUA rats showed the progressively longer latencies across training days, and fewer traversals and shorter durations of the platform in spatial exploration experiments.

Conclusion

DKI imaging was a non-invasive and sensitive method for identifying the HUA-induced hippocampal alterations and cognitive damage.

查看原文本刊更多论文

早期高尿酸血症大鼠海马改变弥散峰度成像的初步研究。

背景：虽然有几种定量方法可用于评估高尿酸血症相关的脑功能障碍，但弥散峰度成像（DKI）对细微的微结构组织变化表现出更高的敏感性，有可能早期发现损伤。目的：探讨高尿酸血症对大鼠DKI治疗认知功能障碍的可行性。材料与方法：将50只雄性大鼠随机分为正常对照组（CON）和高尿酸血症模型组（HUA）。每组随机选取10只大鼠，分别在基线（第0周）和第2、4、6、8周进行DKI纵向扫描。DKI指标通过选择海马区域的双侧对称感兴趣区（ROI）获得，包括平均扩散率（MD）、分数各向异性（FA）和平均峰度（MK）。采用HE染色、GFAP和Iba-1免疫荧光染色观察海马组织病理变化。采用Morris水迷宫（Morris water maze， MWM）测试第8周大鼠的认知能力。结果：本研究中，HUA组海马的MD、FA值呈逐渐下降趋势，而MK值呈逐渐升高趋势。纵向分析显示HUA组和CON组在早期MK差异（第4 -8周）和后期FA差异（第6 -8周）（p ）。结论：DKI成像是一种非侵入性和敏感的方法，用于识别HUA诱导的海马改变和认知损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Magnetic resonance imaging 医学-核医学

CiteScore

4.70

自引率

4.00%

发文量

194

审稿时长

83 days

期刊介绍： Magnetic Resonance Imaging (MRI) is the first international multidisciplinary journal encompassing physical, life, and clinical science investigations as they relate to the development and use of magnetic resonance imaging. MRI is dedicated to both basic research, technological innovation and applications, providing a single forum for communication among radiologists, physicists, chemists, biochemists, biologists, engineers, internists, pathologists, physiologists, computer scientists, and mathematicians.