Unveiling the long non-coding RNA profile of Aeromonas veronii-infected mouse abdominal macrophages

IF 3.6 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2026-11-01 Epub Date: 2026-10-18 DOI:10.1016/j.ygeno.2026.111328

Haichao Song, Meiqing Xu, Jinxiao Feng

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引用次数: 0

Abstract

Long non-coding RNAs (lncRNAs) play key roles in the host's defense against pathogenic infection. Aeromonas veronii is a zoonotic pathogen capable of provoking severe inflammatory responses. However, the function of lncRNAs in A. veronii infected mouse abdominal macrophages (MAMs) remains poorly understood. MAMs serve as the primary defense against bacterial infection and play critical roles in the anti-infectious immune response. Here, we profiled lncRNAs from MAMs infected with A. veronii. RNA sequencing identified a total of 1628 differentially expressed lncRNAs (DElncRNAs), comprising 680 up-regulated and 948 down-regulated genes. An integrated analysis of lncRNA-mRNA interaction networks identified 4888 target genes of DElncRNAs (DETGs). Of these, 55 DElncRNAs were found to be involved in the regulation of seven immune pathways by regulating 41 DETGs. Furthermore, GO and KEGG enrichment analyses revealed that DETGs were significantly enriched in clusters or pathways associated with immune regulation. These findings provide the transcriptomic profile of MAMs. A comprehensive analysis of DElncRNAs in MAMs was conducted in response to A. veronii infection. Co-expression analysis indicates that lncRNAs are serving as vital regulators of immune gene activity through their critical influence on MAMs during A. veronii infection.

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揭示维氏气单胞菌感染小鼠腹腔巨噬细胞的长非编码RNA谱。

长链非编码rna （lncRNAs）在宿主防御致病性感染中发挥关键作用。维罗氏气单胞菌是一种能够引起严重炎症反应的人畜共患病原体。然而，lncrna在veronii感染小鼠腹腔巨噬细胞（MAMs）中的功能仍然知之甚少。MAMs是抵抗细菌感染的主要防御，在抗感染免疫反应中发挥关键作用。在这里，我们分析了感染维罗氏单胞杆菌的MAMs的lncrna。RNA测序共鉴定出1628个差异表达的lncRNAs (DElncRNAs)，包括680个上调基因和948个下调基因。lncRNA-mRNA相互作用网络的综合分析鉴定了4888个delncrna （detg）靶基因。其中，55个delncrna被发现通过调节41个detg参与7种免疫途径的调节。此外，GO和KEGG富集分析显示，detg在与免疫调节相关的簇或途径中显著富集。这些发现提供了MAMs的转录组学特征。对MAMs中的delncrna进行了全面分析，以响应维罗氏单胞杆菌感染。共表达分析表明，lncRNAs在韦氏芽孢杆菌感染期间通过对MAMs的关键影响，作为免疫基因活性的重要调节因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.