MitoNEET, a [2Fe-2S] Protein, Catalyzes Glutathione Oxidation

IF 4.1 3区医学 Q2 CHEMISTRY, MEDICINAL

Chemical Research in Toxicology Pub Date : 2026-10-19 Epub Date: 2026-09-25 DOI:10.1021/acs.chemrestox.6c00367

Abby Jenkins, Tyler Oliver, Cornelius Mbah, Jalyn Jackson, Hannah Skaggs, Jake Caminiti, Henry Aphayasane, Morgan Bonno, Grace Havard, Taylor Bias, Maria Broering, James Montoya, Werner J. Geldenhuys, Michael Menze, Mary Konkle

引用次数: 0

Abstract

MitoNEET is a [2Fe-2S]-containing enzyme proposed to function as a cellular redox-stress sensor. Here we found that purified mitoNEET binds to and directly acts on the cell’s central redox regulator, glutathione (GSH), to its corresponding disulfide (GSSG) without requiring oxygen. The conversion of GSH was monitored by HPLC using an optimized isocratic elution protocol and by spectroscopy with Ellman’s reagent. Additionally, mitoNEET’s novel enzyme activity is considered in the context of reactive electrophiles. Taken together, the discovery of mitoNEET’s GSH reactivity opens the path toward a deeper mechanistic understanding of how mitoNEET senses oxidative stress in the cell.

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[2Fe-2S]蛋白MitoNEET催化谷胱甘肽氧化

MitoNEET是一种含[2Fe-2S]的酶，被认为是细胞氧化还原-应激传感器。在这里，我们发现纯化的mitoneet结合并直接作用于细胞的中央氧化还原调节剂谷胱甘肽（GSH），产生相应的二硫（GSSG），而不需要氧气。采用高效液相色谱法（HPLC）和Ellman试剂法监测谷胱甘肽的转化。此外，mitoNEET的新酶活性是在反应性亲电试剂的背景下考虑的。总之，发现mitoNEET的谷胱甘肽反应性为深入了解mitoNEET如何感知细胞中的氧化应激机制开辟了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Research in Toxicology 医学-毒理学

CiteScore

7.90

自引率

7.30%

发文量

215

审稿时长

3.5 months

期刊介绍： Chemical Research in Toxicology publishes Articles, Rapid Reports, Chemical Profiles, Reviews, Perspectives, Letters to the Editor, and ToxWatch on a wide range of topics in Toxicology that inform a chemical and molecular understanding and capacity to predict biological outcomes on the basis of structures and processes. The overarching goal of activities reported in the Journal are to provide knowledge and innovative approaches needed to promote intelligent solutions for human safety and ecosystem preservation. The journal emphasizes insight concerning mechanisms of toxicity over phenomenological observations. It upholds rigorous chemical, physical and mathematical standards for characterization and application of modern techniques.