Giuseppe Orlando, Ryan S Senger, Amr Sayed Issa, Romina Seifollahi Asl, Alejandra Mena Gutierrez, Colleen C Jay, Emily K E McCracken, Alan C Farney, Robert J Stratta, Christopher J Webb, Hollie B Davis, Tracy Doty, Gwen McPherson, Alexandra R Monetti, John R Savino, Lori Byers, Mohammad Kazem Fallahzadeh, Amber Reeves-Daniel, Natalia V Sakhovskaja, Amish Asthana, Barbara K Yoza, Benedetta Bussolati, Alexei V Mikhailov, Emma Peveri, Christopher L Schaich, Lacey Ngo, Fred Gage, Robert Fisher, John Robertson
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引用次数: 0
Abstract
Objectives: To determine whether Raman spectroscopy-based urine molecular fingerprinting (UMF) may predict delayed graft function (DGF) and assess renal allograft quality prior to transplant and monitor its state of health after transplantation.
Background: Current methods for assessing the quality of the renal allograft at baseline and its state of health post-transplantation are inadequate.
Methods: We conducted a pilot study in 26 patients receiving DCD allografts. UMF was performed on urine samples collected from study participants immediately before transplantation (baseline), on postoperative day 1-3, and monthly thereafter. UMF findings were correlated with clinical and laboratory data. Primary endpoint was identification of a DGF fingerprint. Secondary endpoints were identification of fingerprints for acute rejection, CMV, BK and eGFR.
Results: DGF was observed in 58% of our patients. UMF fingerprints from patients with DGF were distinct from UMF fingerprints of non-DGF patients. Notably, UMF analysis of baseline urine samples from patients prior to transplantation also predicted development of DGF. A correlation of UMF with eGFR was observed, whereas we did not identify fingerprints specific for rejection, CMV or BK possibly due to the small patient population.
Conclusions: In this exploratory study, UMF predicts DGF prior to transplantation and provides information on the state of health of the allograft following transplantation.
期刊介绍：
Clinical Transplantation: The Journal of Clinical and Translational Research aims to serve as a channel of rapid communication for all those involved in the care of patients who require, or have had, organ or tissue transplants, including: kidney, intestine, liver, pancreas, islets, heart, heart valves, lung, bone marrow, cornea, skin, bone, and cartilage, viable or stored.
Published monthly, Clinical Transplantation’s scope is focused on the complete spectrum of present transplant therapies, as well as also those that are experimental or may become possible in future. Topics include:
Immunology and immunosuppression;
Patient preparation;
Social, ethical, and psychological issues;
Complications, short- and long-term results;
Artificial organs;
Donation and preservation of organ and tissue;
Translational studies;
Advances in tissue typing;
Updates on transplant pathology;.
Clinical and translational studies are particularly welcome, as well as focused reviews. Full-length papers and short communications are invited. Clinical reviews are encouraged, as well as seminal papers in basic science which might lead to immediate clinical application. Prominence is regularly given to the results of cooperative surveys conducted by the organ and tissue transplant registries.
Clinical Transplantation: The Journal of Clinical and Translational Research is essential reading for clinicians and researchers in the diverse field of transplantation: surgeons; clinical immunologists; cryobiologists; hematologists; gastroenterologists; hepatologists; pulmonologists; nephrologists; cardiologists; and endocrinologists. It will also be of interest to sociologists, psychologists, research workers, and to all health professionals whose combined efforts will improve the prognosis of transplant recipients.
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