Genetic loci resistant to soybean southern stem canker in Tracy-M and its progeny.

IF 2.4 3区生物学 Q3 GENETICS & HEREDITY

G3: Genes|Genomes|Genetics Pub Date : 2026-10-10 DOI:10.1093/g3journal/jkag278

M Habib Widyawan, James W Buck, Zenglu Li

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引用次数: 0

Abstract

Southern stem canker (SSC), caused by Diaporthe aspalathi, is a major soybean disease causing yield losses of up to 80%. Genetic resistance is the most effective and sustainable strategy for managing SSC. "Tracy-M" is a cultivar with broad resistance to D. aspalathi isolates; however genomic location of the resistance is unknown. To determine the genetic basis of SSC resistance in Tracy-M and its derived progeny, two Tracy-M-derived lines, D85-10404 and D85-10412, were each crossed with a common susceptible parent, G81-2057, to develop recombinant inbred line (RIL) populations. Quantitative Trait Locus (QTL) sequencing (QTL-seq) and composite interval mapping (CIM) identified a common major QTL on chromosome (Chr) 2 in both lines corresponding to Rdm1 locus defined from a previous genetic study. Additionally, second QTL located on Chr 17 (Rdm2 locus) was only detected in the D85-10412-derived RIL population. To validate Tracy-M-derived resistance, an F2:3 population from G00-3213 × Tracy-M was developed. Bulked segregant analysis (BSA) and CIM identified a major QTL on Chr 14, overlapping the previously reported Rdm3 locus in "Crockett" and PI 398469, while Rdm1 was not detected, suggesting genetic background effects. Kompetitive allele-specific PCR (KASP) markers were developed to facilitate selection of Rdm1, Rdm2, and Rdm3 resistance alleles in soybean breeding programs.

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Tracy-M及其后代抗大豆南部茎溃疡病的遗传位点。

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来源期刊

G3: Genes|Genomes|Genetics GENETICS & HEREDITY-

CiteScore

5.10

自引率

3.80%

发文量

305

审稿时长

3-8 weeks

期刊介绍： G3: Genes, Genomes, Genetics provides a forum for the publication of high‐quality foundational research, particularly research that generates useful genetic and genomic information such as genome maps, single gene studies, genome‐wide association and QTL studies, as well as genome reports, mutant screens, and advances in methods and technology. The Editorial Board of G3 believes that rapid dissemination of these data is the necessary foundation for analysis that leads to mechanistic insights. G3, published by the Genetics Society of America, meets the critical and growing need of the genetics community for rapid review and publication of important results in all areas of genetics. G3 offers the opportunity to publish the puzzling finding or to present unpublished results that may not have been submitted for review and publication due to a perceived lack of a potential high-impact finding. G3 has earned the DOAJ Seal, which is a mark of certification for open access journals, awarded by DOAJ to journals that achieve a high level of openness, adhere to Best Practice and high publishing standards.