Tae-Hun Kim, Soonil Kwon, Jaeyong Jung, Jeong Soo Sung, Dong-Ho Han, Min-Jung Kang, Joachim Jose, Misu Lee, Jae-Chul Pyun
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引用次数: 0
Abstract
A ferritin-based influenza A vaccine was developed. The
immunogenic
sequences of the nucleoprotein (NP) were screened using an autodisplayed
Fv-antibody library, and the paratopes with a high-affinity binding
affinity were analyzed through docking simulations. The resulting
antigenic sequences were engineered into single-domain constructs
using a nonimmunogenic linker. These immunogenic sequences were then
expressed in conjunction with ferritin heavy-chain proteins to form
spherical nanoparticles through self-assembly. These NP-ferritin complex
vaccines were subsequently injected into mice, and antibody responses,
cytokine secretion, and T-cell activation profiles were subsequently
analyzed to demonstrate that the ferritin-based NP vaccines activated
innate as well as acquired immune responses.
期刊介绍：
ACS Pharmacology & Translational Science publishes high quality, innovative, and impactful research across the broad spectrum of biological sciences, covering basic and molecular sciences through to translational preclinical studies. Clinical studies that address novel mechanisms of action, and methodological papers that provide innovation, and advance translation, will also be considered. We give priority to studies that fully integrate basic pharmacological and/or biochemical findings into physiological processes that have translational potential in a broad range of biomedical disciplines. Therefore, studies that employ a complementary blend of in vitro and in vivo systems are of particular interest to the journal. Nonetheless, all innovative and impactful research that has an articulated translational relevance will be considered.
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