Enhancement of magnetic biochar on nitrogen removal performance of Anammox granular sludge under acute polystyrene nanoplastics stress

IF 2.7 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of chemical technology and biotechnology Pub Date : 2026-10-09 Epub Date: 2026-07-09 DOI:10.1002/jctb.70239

Jingran Fu, Sishi Liu, Zelong Wang, Shefeng Li, Siyu Ding, Yong Zhang, Xuli Li, Xiaodan Liu, Jing Zhang, Hongyu Wang

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引用次数: 0

Abstract

BACKGROUND

Nanoplastics pose a significant threat to the efficiency and stability of anaerobic ammonium oxidation (Anammox). This study investigated the nitrogen removal efficiency, extracellular polymeric substance (EPS) secretion, microbial community, and enhancement mechanisms of Anammox with magnetic biochar (MBC) under sudden exposure to high-concentration polystyrene nanoplastics (PS-NPs).

RESULTS

Results demonstrated that sudden exposure to PS-NPs at 300 mg L⁻¹ distinctly reduced the nitrogen removal efficiency of Anammox, while the addition of MBC could significantly alleviate the inhibition. Fourier transform infrared analysis manifested that MBC could adsorb the PS-NPs by electrostatic attraction, surface complexation and π–π interactions. MBC could stimulate anaerobic ammonium-oxidizing bacteria to secrete a large amount of EPS (including protein, humic acids, and cytochrome c). Microbial community analysis showed that the addition of MBC could maintain the stability of microbial community structure. KEGG pathway analysis indicated that addition of MBC significantly enhanced the nitrogen metabolism pathway and glycan biosynthesis and metabolism pathway of Anammox granular sludge (AnGS) under acute PS-NPs stress, thereby improving its nitrogen removal efficiency and structural stability.

CONCLUSION

This study provided valuable insights into the response characteristics and enhancement mechanism of MBC for AnGS exposed to PS-NPs, which may be helpful for the application of Anammox in actual wastewater treatment. © 2026 Society of Chemical Industry (SCI).

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磁性生物炭对急性聚苯乙烯纳米塑料应力下厌氧氨氧化颗粒污泥脱氮性能的增强

纳米塑料对厌氧氨氧化（Anammox）的效率和稳定性构成了重大威胁。研究了突然暴露于高浓度聚苯乙烯纳米塑料（PS-NPs）下，磁性生物炭（MBC）对厌氧氨氧化反应的脱氮效率、胞外聚合物（EPS）分泌、微生物群落以及强化机制。结果表明，突然暴露于300 mg L−1的PS-NPs明显降低了厌氧氨氧化菌的脱氮效率，而MBC的加入可以显著缓解这种抑制作用。红外傅里叶变换分析表明，MBC可以通过静电吸引、表面络合和π -π相互作用吸附PS-NPs。MBC能刺激厌氧氨氧化菌分泌大量EPS（包括蛋白质、腐植酸和细胞色素c）。微生物群落分析表明，添加MBC可以保持微生物群落结构的稳定性。KEGG途径分析表明，MBC的添加显著增强了急性PS-NPs胁迫下Anammox颗粒污泥（AnGS）的氮代谢途径和聚糖生物合成代谢途径，从而提高了其脱氮效率和结构稳定性。结论本研究揭示了MBC对暴露于PS-NPs环境下的AnGS的响应特征和增强机制，为厌氧氨氧化在实际废水处理中的应用提供了参考。©2026化学工业学会（SCI）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of chemical technology and biotechnology 工程技术-工程：化工

CiteScore

7.00

自引率

5.90%

发文量

268

审稿时长

1.7 months

期刊介绍： Journal of Chemical Technology and Biotechnology(JCTB) is an international, inter-disciplinary peer-reviewed journal concerned with the application of scientific discoveries and advancements in chemical and biological technology that aim towards economically and environmentally sustainable industrial processes.