Astragalin From Camellia sinensis Flowers Increases Collagen Content Alongside Autophagy-Related Mitochondrial Metabolic Changes in Caenorhabditis elegans.

IF 4.1 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Food Science Pub Date : 2026-10-01 DOI:10.1111/1750-3841.71503

Qiannan Pan, Kejie Peng, Binhai Shi, Daoxin Dai, Yansong Zhang, Zhangtie Wang, Baiyi Lu

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Abstract

Collagen, the predominant structural protein in skin, is essential for maintaining elasticity and delaying aging. With age-related collagen depletion, enhancing its biosynthesis, particularly via natural bioactive compounds, has attracted scientific and commercial attention. Here, we investigated the collagen-promoting activity of edible flowers by assessing their matrix metalloproteinase (MMP) inhibition and collagen content change. Among 28 tested flower extracts, Camellia sinensis (CSK) exhibited the strongest MMP inhibition (>75%) and significantly increased collagen content in Caenorhabditis elegans (C. elegans). UHPLC-QE-MS analysis identified astragalin, a polyphenol, as the key active compound, which outperformed controls (vitamin C, VC) by elevating collagen levels via mitophagy activation, adenosine triphosphate/nicotinamide adenine dinucleotide phosphate (ATP/NADPH) modulation, and oxidative stress (reactive oxygen species, ROS) reduction. Pharmacological inhibition of autophagy with chloroquine attenuated the astragalin-associated increase in collagen content. In conclusion, our results support that CSK extract and its active constituent astragalin promote collagen production through mitophagy-mediated NADPH metabolic regulation, offering a novel mechanism of collagen enhancement and highlighting the potential of plant-derived polyphenols in dermatological research.

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茶花黄芪苷增加秀丽隐杆线虫自噬相关线粒体代谢变化及胶原含量

胶原蛋白是皮肤中主要的结构蛋白，对保持皮肤弹性和延缓衰老至关重要。随着与年龄相关的胶原蛋白消耗，增强其生物合成，特别是通过天然生物活性化合物，已经引起了科学界和商界的关注。本文通过考察食用花基质金属蛋白酶（MMP）抑制和胶原蛋白含量的变化，研究了食用花的促胶原活性。在28种被试花提取物中，山茶提取物（Camellia sinensis， CSK）对秀丽隐杆线虫（C. elegans）的MMP抑制作用最强（>75%），并显著提高胶原蛋白含量。UHPLC-QE-MS分析发现，黄芪甲苷是一种多酚类化合物，它通过激活线粒体自噬、三磷酸腺苷/烟酰胺腺嘌呤二核苷酸磷酸（ATP/NADPH）调节和氧化应激（活性氧，ROS）减少来提高胶原蛋白水平，从而优于对照（维生素C， VC）。用氯喹对自噬进行药理学抑制，可减弱黄芪黄苷相关的胶原蛋白含量增加。综上所述，我们的研究结果支持CSK提取物及其活性成分黄芪甲苷通过线粒体自噬介导的NADPH代谢调节促进胶原生成，提供了一种新的胶原增强机制，并突出了植物源性多酚在皮肤病学研究中的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.