Matrix-Assisted Laser Desorption/Ionization Imaging Mass Spectrometry.

Abstract

Matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) is a powerful molecular imaging technology that is capable of imaging a wide range of molecular classes at cellular resolution. This Primer includes an overview of basic imaging mass spectrometry concepts, instrumentation, processing approaches, and advanced applications. We also address key challenges and considerations with an eye on how to optimize instrumentation and methods to overcome issues with spatial resolution, sensitivity, and specificity.