Diagnosis of bovine genital leptospirosis in asymptomatic bulls: Comparison of methods and samples under field conditions.

IF 0.9 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Record Pub Date : 2026-09-27 DOI:10.1002/vetr.71220

Juliana Pedrosa, Daiany Motta, Wilmara Gregg, André Rodrigues, Walter Lilenbaum

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引用次数: 0

Abstract

Background: Bovine leptospirosis is a zoonosis of global importance that is associated with reproductive losses. Although diagnosis in females has advanced, genital infection in males remains poorly understood. This cross-sectional study aimed to compare diagnostic methods and sample types for detecting bovine genital leptospirosis (BGL) in bulls under field conditions.

Methods: Blood, urine, semen and preputial cytology samples were collected from 37 unvaccinated bulls and analysed by serology (microscopic agglutination test -MAT) and lipL32-PCR.

Results: Serology showed low seroreactivity (13.5%), whereas PCR detected infection in 80.5% of bulls. Leptospira DNA was identified in semen (70.6%), preputial cytology samples (50%) and urine (46.8%). PCR detected a higher proportion of positive animals than MAT (p < 0.05). The genital tract had a higher frequency of Leptospira detection than the urinary tract (p < 0.05), with no significant difference between semen and preputial cytology samples (p > 0.05).

Limitations: The sample size was small, and the number of samples collected differed among sample types.

Conclusions: PCR using genital samples-semen and/or preputial cytology-is the method of choice for diagnosing BGL in asymptomatic bulls.

查看原文本刊更多论文

无症状公牛中牛生殖器钩端螺旋体病的诊断：在野外条件下方法和样本的比较。

背景：牛钩端螺旋体病是一种全球重要的人畜共患病，与生殖损失有关。尽管对女性的诊断已取得进展，但对男性生殖器感染的了解仍然很少。本横断面研究旨在比较在野外条件下检测牛生殖器钩端螺旋体病（BGL）的诊断方法和样本类型。方法：采集37头未接种疫苗公牛的血液、尿液、精液和包皮细胞学样本，采用血清学（显微凝集试验-MAT）和lipL32-PCR进行分析。结果：血清学显示低血清反应性（13.5%），而PCR检测感染率为80.5%。精液（70.6%）、包皮细胞学样本（50%）和尿液（46.8%）检出钩端螺旋体DNA。PCR检测阳性动物比例高于MAT （p < 0.05）。生殖道钩端螺旋体检出率高于泌尿道（p < 0.05），精液和包皮细胞学检查差异无统计学意义（p < 0.05）。局限性：样本量较小，样本量因样本类型而异。结论：使用生殖器样本（精液和/或包皮细胞学）进行PCR是诊断无症状公牛BGL的首选方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Record 农林科学-兽医学

CiteScore

2.10

自引率

9.10%

发文量

1181

审稿时长

6-12 weeks

期刊介绍： Veterinary Record (branded as Vet Record) is the official journal of the British Veterinary Association (BVA) and has been published weekly since 1888. It contains news, opinion, letters, scientific reviews and original research papers and communications on a wide range of veterinary topics, along with disease surveillance reports, obituaries, careers information, business and innovation news and summaries of research papers in other journals. It is published on behalf of the BVA by BMJ Group.