{"title":"Cytological Assessment of Maize Pollen Viability Using a Simplified Staining Protocol.","authors":"Mateusz Zelkowski, Wojciech P Pawlowski","doi":"10.1101/pdb.prot108742","DOIUrl":null,"url":null,"abstract":"<p><p>Anomalies of meiosis frequently result in abnormal chromosome segregation, which leads to defects in pollen formation in maize. Thus, assessing pollen viability is an important measure for examining the overall success of male sexual reproduction. Pollen viability tests are used to characterize mutants defective in meiosis and microsporogenesis, and to determine the effects of genome instability and environmental conditions on reproduction. This protocol describes a rapid method for assessing pollen viability in maize, using a simplified cytological staining approach. Traditional pollen staining methods, such as Alexander staining, often require hazardous chemicals that are increasingly restricted due to safety concerns. The method presented here uses easily accessible reagents and avoids highly toxic substances.</p>","PeriodicalId":10496,"journal":{"name":"Cold Spring Harbor protocols","volume":" ","pages":"pdb.prot108742"},"PeriodicalIF":0.0000,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cold Spring Harbor protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1101/pdb.prot108742","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Anomalies of meiosis frequently result in abnormal chromosome segregation, which leads to defects in pollen formation in maize. Thus, assessing pollen viability is an important measure for examining the overall success of male sexual reproduction. Pollen viability tests are used to characterize mutants defective in meiosis and microsporogenesis, and to determine the effects of genome instability and environmental conditions on reproduction. This protocol describes a rapid method for assessing pollen viability in maize, using a simplified cytological staining approach. Traditional pollen staining methods, such as Alexander staining, often require hazardous chemicals that are increasingly restricted due to safety concerns. The method presented here uses easily accessible reagents and avoids highly toxic substances.
Cold Spring Harbor protocolsBiochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.00
自引率
0.00%
发文量
163
期刊介绍：
Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.
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