Optical Ultramicroscopy of Nanoparticles in Aqueous Solutions Using Elastic Light Scattering: Real-World Capabilities and Comparison with TEM and DLS

IF 1.1 4区物理与天体物理 Q3 PHYSICS, MULTIDISCIPLINARY

Physics of Wave Phenomena Pub Date : 2026-09-09 DOI:10.3103/S1541308X26600091

G. O. Silaev, O. V. Morozova, V. N. Krasheninnikov, F. V. Vereshchagin, V. Z. Lankin, Yu. G. Vainer

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Abstract

An improved optical ultramicroscope is described to be used for visualizing single nanoparticles (NPs) sized over 5–10 nm in aqueous solutions and determining their hydrodynamic radii. The ultramicroscope is based on detection of Brownian motion trajectories of NPs using the elastic light scattering signals of two—laser and nonlaser—sources and their subsequent analysis for determining individual hydrodynamic radii of the detected NPs. The actual capabilities of the developed microscope in studying NPs of a different nature, including biological ones, are demonstrated. The analysis results for silver and silicon dioxide (SiO₂) NPs obtained using the developed microscope are compared to those obtained using a transmission electron microscope and a dynamic light scattering microscope (DLS). The developed microscope is shown to hold promise in medical diagnosis applications. Advantages of using a nonlaser source of bright incoherent radiation for analysis of submicron particles larger than 100 nm are demonstrated.

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使用弹性光散射的光学超微显微镜在水溶液中观察纳米粒子：真实世界的能力和与TEM和DLS的比较

本文描述了一种改进的光学超显微镜，用于在水溶液中可视化大小超过5-10纳米的单纳米颗粒（NPs），并确定它们的流体动力学半径。超显微镜是基于利用双激光和非激光源的弹性光散射信号检测NPs的布朗运动轨迹及其随后的分析来确定检测到的NPs的单个流体动力半径。开发的显微镜在研究不同性质的NPs的实际能力，包括生物的，被证明。并与透射电子显微镜和动态光散射显微镜（DLS）的分析结果进行了比较。所研制的显微镜在医学诊断方面具有广阔的应用前景。在分析大于100 nm的亚微米粒子时，证明了使用非激光源的非相干辐射的优点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Physics of Wave Phenomena PHYSICS, MULTIDISCIPLINARY-

CiteScore

2.50

自引率

21.40%

发文量

审稿时长

>12 weeks

期刊介绍： Physics of Wave Phenomena publishes original contributions in general and nonlinear wave theory, original experimental results in optics, acoustics and radiophysics. The fields of physics represented in this journal include nonlinear optics, acoustics, and radiophysics; nonlinear effects of any nature including nonlinear dynamics and chaos; phase transitions including light- and sound-induced; laser physics; optical and other spectroscopies; new instruments, methods, and measurements of wave and oscillatory processes; remote sensing of waves in natural media; wave interactions in biophysics, econophysics and other cross-disciplinary areas.