Pseudo-hypouricemia in four patients with lymphoma and one patient with breast cancer: Interference analysis and elimination.

IF 1.1 4区医学 Q3 MEDICAL LABORATORY TECHNOLOGY

Annals of Clinical Biochemistry Pub Date : 2026-08-18 DOI:10.1177/00045632261478795

Guanqiao Shen, Qi Zhang, Shunchang Li

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Abstract

ObjectiveTo investigate the interfering factors and correction methods in the determination of serum uric acid (UA) using the uricase method in patients with elevated immunoglobulin M (IgM).Materials and MethodsIn clinical practice, the UA levels of five patients with elevated IgM were measured using the Beckman AU5800 analyzer with its reagents or Zhongyuan reagents. The reaction curves were analyzed. Three methods dilution with normal saline, dilution with saturated saline, and polyethylene glycol 6000 (PEG 6000) precipitation were applied to eliminate interference. For comparison, UA was also measured using the Abbott Ci8200 analyzer with its reagents.ResultsThe serum IgM levels for cases 1 to 5 were 12.39, 27.0, 12.09, 7.33, and 11.05 g/L, respectively. The initial UA results obtained with the Beckman AU5800 and its reagents were 48.4, 101.3, 96.6, 0.6, and 66.6 μmol/L. All five cases exhibited abnormal biochemical reaction curves. After treatment with normal saline, samples 1 and 5 yielded negative values, while after saturated saline treatment, sample 5 still showed a negative value and sample 1 showed an extremely low value, indicating incomplete correction of the interference. In contrast, the PEG precipitation method markedly improved the UA results, and the obtained values were comparable to those from the Abbott system.ConclusionThe PEG precipitation method provides superior correction compared to dilution with normal or saturated saline and yields results that are closer to the patient's true value.

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4例淋巴瘤和1例乳腺癌假性低尿酸血症：干扰分析与消除。

目的探讨尿酶法测定免疫球蛋白M （IgM）升高患者血清尿酸（UA）的干扰因素及校正方法。材料与方法在临床实践中，采用Beckman AU5800型血药分析仪，用其试剂或中原试剂检测5例IgM升高患者的UA水平。分析了反应曲线。采用生理盐水稀释、饱和盐水稀释、聚乙二醇6000 （PEG 6000）沉淀三种方法消除干扰。为了比较，UA也使用雅培Ci8200分析仪及其试剂进行测量。结果病例1 ~ 5血清IgM水平分别为12.39、27.0、12.09、7.33、11.05 g/L。使用Beckman AU5800及其试剂获得的初始UA值分别为48.4、101.3、96.6、0.6和66.6 μmol/L。5例患者均出现异常生化反应曲线。经生理盐水处理后，样品1和样品5均为负值，而经饱和盐水处理后，样品5仍为负值，样品1为极低值，说明干扰校正不完全。相比之下，聚乙二醇沉淀法显著改善了UA结果，所得值与雅培系统相当。结论与正常或饱和生理盐水稀释相比，聚乙二醇沉淀法提供了更好的校正，并且产生的结果更接近患者的真实值。

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来源期刊

Annals of Clinical Biochemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

5.20

自引率

4.50%

发文量

期刊介绍： Annals of Clinical Biochemistry is the fully peer reviewed international journal of the Association for Clinical Biochemistry and Laboratory Medicine. Annals of Clinical Biochemistry accepts papers that contribute to knowledge in all fields of laboratory medicine, especially those pertaining to the understanding, diagnosis and treatment of human disease. It publishes papers on clinical biochemistry, clinical audit, metabolic medicine, immunology, genetics, biotechnology, haematology, microbiology, computing and management where they have both biochemical and clinical relevance. Papers describing evaluation or implementation of commercial reagent kits or the performance of new analysers require substantial original information. Unless of exceptional interest and novelty, studies dealing with the redox status in various diseases are not generally considered within the journal''s scope. Studies documenting the association of single nucleotide polymorphisms (SNPs) with particular phenotypes will not normally be considered, given the greater strength of genome wide association studies (GWAS). Research undertaken in non-human animals will not be considered for publication in the Annals. Annals of Clinical Biochemistry is also the official journal of NVKC (de Nederlandse Vereniging voor Klinische Chemie) and JSCC (Japan Society of Clinical Chemistry).