Ayse Mine Saridag, Isik Didem Karagoz, Sebastian Wachsmann-Hogiu, Mehmet Kahraman
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引用次数: 0
Abstract
In this study, we used flexible and inexpensive diatomite/AgNPs strips and Layer-by-Layer (LbL) assembly to develop sandwich assays for the detection of cancer biomarkers of interest via Surface-enhanced Raman scattering (SERS). The modified SERS active strips were used to capture antigens of interest, incubate Raman probes, and generate SERS maps based on specific Raman peaks of the Raman probes. Simultaneous detection and quantification of multiple biomarkers, including HER2, CA15-3, CA27-29, PSA, and MUC4, were demonstrated by the immobilization of specific antibodies and the use of uniquely labeled Raman probes. Spatially resolved and co-immobilized multiplexing strategies were developed and successfully validated, showing high specificity and sensitivity with negligible background interference from serum components. The flexible and cost-effective nature of the diatomite/AgNPs substrate, combined with its strong SERS enhancement, offers significant advantages over traditional immunoassay techniques.
期刊介绍：
ChemPhysChem is one of the leading chemistry/physics interdisciplinary journals (ISI Impact Factor 2018: 3.077) for physical chemistry and chemical physics. It is published on behalf of Chemistry Europe, an association of 16 European chemical societies.
ChemPhysChem is an international source for important primary and critical secondary information across the whole field of physical chemistry and chemical physics. It integrates this wide and flourishing field ranging from Solid State and Soft-Matter Research, Electro- and Photochemistry, Femtochemistry and Nanotechnology, Complex Systems, Single-Molecule Research, Clusters and Colloids, Catalysis and Surface Science, Biophysics and Physical Biochemistry, Atmospheric and Environmental Chemistry, and many more topics. ChemPhysChem is peer-reviewed.
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