Modulation of X-irradiation-induced γ-H2AX focus stability in human blood lymphocytes: A technical note

IF 2.8 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2026-08-01 Epub Date: 2026-08-08 DOI:10.1016/j.mrgentox.2026.503957

Anushka Regil, Revanth Shankar, Divya K. Mohan, Venkateswarlu Raavi, Venkatachalam Perumal

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引用次数: 0

Abstract

Histones, essential building blocks of the nucleosome, undergo dynamic changes during the cell cycle. Phosphorylation of histone protein H2AX occurs at sites of DNA double-strand breaks (DSBs) and is a sensitive marker of DNA damage. Detection of phosphorylated H2AX, the γ-H2AX assay, has been widely used in experimental models, including human tissues and cell lines. The assay has been used in research on DNA repair, drug development, genotoxicity assessment, radiation biology, diagnostic radiology, and radiotherapy protocol optimization. Among these applications, the γ-H2AX assay has gained particular attention in radiation biodosimetry due to its rapid turnaround time. Despite its potential for radiation triage, the assay's utility in cases of delayed sample collection/processing remains limited, because quantitation is constrained by rapid dephosphorylation, catalyzed by phosphatases such as Protein Phosphatase 2 A (PP2A). Potential stabilizing agents, such as fostriecin, okadaic acid, calyculin A, and LiCl, have been suggested to preserve γ-H2AX by modulating phosphatase activity. We evaluated the possible stabilising effects of calyculin A and LiCl on γ-H2AX foci in irradiated human blood lymphocytes. Pre-treatment with either calyculin A (10 nM) or LiCl (20 mM) yielded higher levels of γ-H2AX foci, 0.5 h post-X-irradiation; however, the stability of the γ-H2AX foci declined, 24 h post-irradiation.

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x照射诱导的人血液淋巴细胞γ-H2AX聚焦稳定性的调节：技术说明

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.