Catalytic Activity and Stability of Alkaline Phosphatase and β-Galactosidase on Aluminosilicate Adsorbents

IF 1.1 4区工程技术 Q3 CHEMISTRY, ORGANIC

Petroleum Chemistry Pub Date : 2026-04-07 DOI:10.1134/S0965544126600268

Larisa F. Atyaksheva, Artur Yu. Fastov, Sergey A. Fastov, Daniil A. Fedosov

引用次数: 0

Abstract

This study investigates the catalytic activity and thermal stability of two enzymes—alkaline phosphatase and β-galactosidase—immobilized on the surface of BEA and MFI-type zeolites, as well as on natural aluminosilicate halloysite nanotubes (HNTs). The catalytic performance parameters (activity and Michaelis constant) were evaluated for all prepared catalysts. Thermal inactivation of the enzymes on the adsorbent surfaces was studied at 50, 55, and 60°C, and the first-order effective rate constants for inactivation (denaturation) and the corresponding activation energies were determined. The adsorbed enzyme layers partially retained their catalytic activity, while their thermal stability was significantly enhanced.

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碱性磷酸酶和β-半乳糖苷酶在铝硅酸盐吸附剂上的催化活性和稳定性

本研究考察了碱性磷酸酶和β-半乳糖苷酶两种酶在BEA型和mfi型沸石表面以及天然铝硅酸盐高岭土纳米管（HNTs）上的催化活性和热稳定性。对制备的催化剂的催化性能参数（活性和米切里斯常数）进行了评价。在50、55和60℃条件下，研究了酶在吸附剂表面的热失活，并确定了失活（变性）的一级有效速率常数和相应的活化能。吸附酶层部分保留了催化活性，热稳定性显著增强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Petroleum Chemistry 工程技术-工程：化工

CiteScore

2.50

自引率

21.40%

发文量

102

审稿时长

6-12 weeks

期刊介绍： Petroleum Chemistry (Neftekhimiya), founded in 1961, offers original papers on and reviews of theoretical and experimental studies concerned with current problems of petroleum chemistry and processing such as chemical composition of crude oils and natural gas liquids; petroleum refining (cracking, hydrocracking, and catalytic reforming); catalysts for petrochemical processes (hydrogenation, isomerization, oxidation, hydroformylation, etc.); activation and catalytic transformation of hydrocarbons and other components of petroleum, natural gas, and other complex organic mixtures; new petrochemicals including lubricants and additives; environmental problems; and information on scientific meetings relevant to these areas. Petroleum Chemistry publishes articles on these topics from members of the scientific community of the former Soviet Union.