The impacts of native potential probiotic cocktail to prevent or ameliorate inflammation by targeting autophagy signalling pathway.

Abstract

Intestinal inflammation can lead to inflammation-related diseases such as IBD, so modulation of inflammatory responses is crucial for maintaining homeostasis in the gut and alleviating intestinal inflammation. Autophagy may play a functional role in inflammatory responses and inflammatory signalling pathways. In addition, probiotics can influence important signalling pathways that lead to inflammation. Thus, our objective was to investigate the impact of probiotics on autophagy gene expression to prevent or reduce inflammation by targeting the autophagy signalling pathway. A relative real-time PCR assay was performed to evaluate gene expression involved in the autophagy process after exposing the HT-29 cell line to sonicated pathogens and adding a native potent Lactobacillus/Bifidobacterium cocktail before, after, and simultaneously with inflammation. In general, the native potential Lactobacillus/Bifidobacterium mixture was able to significantly increase autophagy gene expression in all phases and our selected probiotics can influence all phases of the autophagy signalling pathway. Our native Lactobacillus/Bifidobacterium cocktail exhibited a significant impact on the expression of autophagy genes throughout all treatments, particularly during the pre-inflammatory phase. Consequently, our selected probiotics can potentially serve a preventive function in an in vitro model of inflammation.