Data of annotated draft genome assemblies of three bacterial isolates from the ‘Panchabrihi’ rice, soybean, and bean-associated niches in Bangladesh

IF 1.9 Q3 MULTIDISCIPLINARY SCIENCES

Data in Brief Pub Date : 2026-08-01 Epub Date: 2026-07-16 DOI:10.1016/j.dib.2026.113081

Nusrat Sultana, Abed Chaudhury, Saria Farheen

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引用次数: 0

Abstract

We have collected the bacterial communities inhabiting the rhizosphere and seed-surface of selected crop plants (‘Panchabrihi’ rice, soybean, and bean) in Bangladesh and created a draft genome assembly database of the isolated bacterial strains. Three bacterial isolates were : A1 from the rhizosphere of Panchabrihi rice, A7 and A10 from the surface of soybean and bean seeds. Whole-genome sequencing, draft genome assembly, BLAST comparison with reference genomes, Nucleotide Identity (ANI), digital DNA-DNA hybridization (dDDH), Average Amino Acid Identity (AAI), phylogenomic analyses, and annotations using Bakta revealed that the isolates are Bacillus cereus A1, Bacillus subtilis A7, and Acinetobacter soli A10. Paired-end Illumina sequencing data, draft genome assembly, and annotations generated were deposited in the NCBI database under BioProject PRJNA1366070. Afterwards, we have analysed the potential of plant growth–promoting (PGP) capacities using the eggNOG-mapper analysis, COG functional classification, secondary metabolite biosynthetic gene cluster analysis using antiSMASH, and KEGG Orthology (KO) completeness analysis. After manual curation of the functional properties of the predicted genes, we found that all three genomes were associated with PGP functions, including nutrient solubilization, phytohormone production, stress tolerance, and root colonization. Additionally, we have also analysed the antibiotic resistance genes (ARGs), mobile genetic elements, virulence-associated genes, toxin genes, and pathogenicity genes of the isolated strains, and generated the respective database. The draft genomes were also found to have several antibiotic resistance determinant genes, mobile genetic elements, virulence-associated genes, and human pathogenic genes. Therefore, we have performed a comparative biosafety risk assessment on all three genomes and concluded that Bacillus subtilis A7 (rather than the A1 and A10) will be a safer option for possible sustainable agricultural practices. We have generated a comprehensive metadata table and submitted all relevant databases to Zenodo. Our databases will be useful for future identification of the potential PGP bacterial strains and for assessing the biosafety traits before agricultural application.

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来自孟加拉国“Panchabrihi”水稻、大豆和豆类相关生态位的三种细菌分离物的注释草图基因组组装数据

我们收集了孟加拉国选定作物（“Panchabrihi”水稻、大豆和豆类）的根际和种子表面的细菌群落，并创建了分离菌株的基因组组装数据库草案。3株分离菌株分别为：A1来自水稻根际，A7和A10来自大豆和大豆种子表面。全基因组测序、基因组草图汇编、BLAST与参考基因组比较、核苷酸鉴定（ANI）、数字DNA-DNA杂交（dDDH）、平均氨基酸鉴定（AAI）、系统基因组分析和Bakta注释显示，分离物为蜡样芽孢杆菌A1、枯草芽孢杆菌A7和土壤不动杆菌A10。配对端Illumina测序数据、基因组组装草图和生成的注释存储在BioProject PRJNA1366070下的NCBI数据库中。随后，我们利用eggNOG-mapper分析、COG功能分类、使用antiSMASH的次生代谢物生物合成基因聚类分析和KEGG Orthology （KO）完整性分析分析了植物促生长（PGP）能力的潜力。在对预测基因的功能特性进行人工管理后，我们发现所有三个基因组都与PGP功能相关，包括营养溶解、植物激素产生、胁迫耐受性和根定植。此外，我们还分析了分离菌株的抗生素耐药基因（ARGs）、移动遗传元件、毒力相关基因、毒素基因和致病性基因，并建立了相应的数据库。初步基因组还发现了几种抗生素耐药性决定基因、移动遗传元件、毒力相关基因和人类致病基因。因此，我们对所有三个基因组进行了比较生物安全风险评估，并得出结论，枯草芽孢杆菌A7（而不是A1和A10）将是可能的可持续农业实践中更安全的选择。我们已经生成了一个全面的元数据表，并将所有相关数据库提交给Zenodo。我们的数据库将有助于未来鉴定潜在的PGP菌株，并在农业应用前评估其生物安全特性。

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来源期刊

Data in Brief MULTIDISCIPLINARY SCIENCES-

CiteScore

3.10

自引率

0.00%

发文量

996

审稿时长

70 days

期刊介绍： Data in Brief provides a way for researchers to easily share and reuse each other''s datasets by publishing data articles that: -Thoroughly describe your data, facilitating reproducibility. -Make your data, which is often buried in supplementary material, easier to find. -Increase traffic towards associated research articles and data, leading to more citations. -Open up doors for new collaborations. Because you never know what data will be useful to someone else, Data in Brief welcomes submissions that describe data from all research areas.