Comparative analysis of the complete multi-chromosomal mitogenome of an antitussive herb Fritillaria unibracteata.

IF 3.9 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

BMC Genomics Pub Date : 2026-07-15 DOI:10.1186/s12864-026-13193-w

Song Lu

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引用次数: 0

Abstract

Background: A renowned antitussive herb, Fritillaria unibracteata P. K. Hsiao & K. C. Hsia, has long been used as the original source of many traditional Chinese medicines (TCM) prescriptions for its pharmacological effects. Currently, this species is at risk of extinction owing to overexploitation. Genomic data can provide a scientific basis for the effective protection of wild germplasm resources. Although the plastome of F. unibracteata has been released, its mitogenome remains unclear.

Results: The mitogenome of F. unibracteata exhibited a sophisticated multi-chromosomal configuration containing one master circular chromosome (82,085 bp) and 21 submaster chromosomes (18,718 bp-54,256 bp). It contained 60 genes, including 44 PCGs, 13 tRNA and 3 rRNA genes. Repeat sequences, including 862 dispersed repeats, 142 simple repeats, and 23 tandem repeats, contributed more to the mitogenome size than coding genes and chloroplast-derived fragments. A total of 518 RNA editing sites were identified in 36 PCGs, with NADH dehydrogenase and cytochrome c biogenesis genes being the most prevalent. The codon usage bias is in accordance with the model for most terrestrial plants that use ATG as an initiator codon and shows an obvious A/T (U) bias in the third site of all PCGs. These results provide key insights into the F. unibracteata mitogenome.

Conclusion: The assembled and annotated F. unibracteata mitogenome advanced the features and organization of multi-chromosome genomes. This provides helpful information for further research on conservation, breeding, phylogenetic, and evolutionary studies of the genus Fritillaria.

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止咳草药贝母多染色体有丝分裂全基因组的比较分析。

背景：贝母是一种著名的止咳草药，因其药理作用，长期以来一直被用作许多中药处方的原始来源。目前，由于过度开发，该物种面临灭绝的危险。基因组数据可为有效保护野生种质资源提供科学依据。尽管独角兽的质体已被释放，但其有丝分裂基因组尚不清楚。结果：独角仙有丝分裂基因组呈现出复杂的多染色体结构，包含1条主环状染色体（82,085 bp）和21条亚主染色体（18,718 bp-54,256 bp）。包含60个基因，其中PCGs基因44个，tRNA基因13个，rRNA基因3个。重复序列，包括862个分散重复序列，142个简单重复序列和23个串联重复序列，比编码基因和叶绿体来源片段对有丝分裂基因组大小的贡献更大。在36个PCGs中共鉴定出518个RNA编辑位点，其中以NADH脱氢酶和细胞色素c生物发生基因最为普遍。密码子使用偏倚与大多数陆生植物使用ATG作为启动密码子的模式一致，并且在所有PCGs的第三位点显示出明显的A/T (U)偏倚。这些结果为F. unbracteata有丝分裂基因组提供了关键的见解。结论：组装和注释的独角龙葵有丝分裂基因组促进了多染色体基因组的特征和组织。这为贝母属植物的保护、育种、系统发育和进化研究提供了有益的信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Genomics 生物-生物工程与应用微生物

CiteScore

7.40

自引率

4.50%

发文量

769

审稿时长

6.4 months

期刊介绍： BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.