Fecal gas time curve analysis for health monitoring.

IF 2.7 4区 医学 Q1 BIOCHEMICAL RESEARCH METHODS
Ju-Young Park, Su-Hyeon Park, Jung-Ho Lee, Young-Chun Park
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Abstract

Human feces are metabolic waste products that emit a wide range of volatile organic compounds, the result of dynamic interactions between gut microbiota and the host physiology. Fecal gas profiles are recognized as potential indicators of an individual's health status; however, most analyses rely on expensive laboratory equipment such as gas chromatography. In contrast, studies utilizing low-cost gas sensors for stool gas analysis remain limited, partly due to the high sensitivity of gas concentration data to environmental variables such as stool weight and ambient conditions. In this study, gas concentration data (ppm) were converted into normalized gas reduction ratio curves (%), referred to here as time curves, so that temporal gas-release patterns could be compared across samples with reduced influence from absolute signal differences. Fifteen stool samples were collected from four individuals and analyzed according to the Bristol stool scale. Time curves corresponding to the same Bristol stool type showed reproducible convergence into representative temporal profiles, where different stool types exhibited distinguishable gas-decay patterns. These findings suggest that time curve analysis may be useful for characterizing stool-type-related temporal gas patterns while reducing the influence of environmental variability in low-cost fecal gas sensing.

用于健康监测的粪便时间曲线分析。
人类粪便是代谢废物,释放出多种挥发性有机化合物,是肠道微生物群与宿主生理动态相互作用的结果。粪便气体分布被认为是个人健康状况的潜在指标;然而,大多数分析依赖于昂贵的实验室设备,如气相色谱法。相比之下,利用低成本气体传感器进行粪便气体分析的研究仍然有限,部分原因是气体浓度数据对粪便重量和环境条件等环境变量的敏感性很高。在本研究中,将气体浓度数据(ppm)转换为归一化气体还原比曲线(%),这里称为时间曲线,以便在减少绝对信号差异影响的情况下,比较样品间的时间气体释放模式。从4个人身上收集了15份粪便样本,并根据布里斯托尔粪便量表进行了分析。同一种布里斯托大便类型对应的时间曲线可重复收敛为具有代表性的时间剖面,其中不同大便类型表现出可区分的气体衰变模式。这些发现表明,时间曲线分析可能有助于表征与粪便类型相关的时间气体模式,同时减少低成本粪便气体传感中环境变化的影响。
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来源期刊
Journal of breath research
Journal of breath research BIOCHEMICAL RESEARCH METHODS-RESPIRATORY SYSTEM
CiteScore
7.60
自引率
21.10%
发文量
49
审稿时长
>12 weeks
期刊介绍: Journal of Breath Research is dedicated to all aspects of scientific breath research. The traditional focus is on analysis of volatile compounds and aerosols in exhaled breath for the investigation of exogenous exposures, metabolism, toxicology, health status and the diagnosis of disease and breath odours. The journal also welcomes other breath-related topics. Typical areas of interest include: Big laboratory instrumentation: describing new state-of-the-art analytical instrumentation capable of performing high-resolution discovery and targeted breath research; exploiting complex technologies drawn from other areas of biochemistry and genetics for breath research. Engineering solutions: developing new breath sampling technologies for condensate and aerosols, for chemical and optical sensors, for extraction and sample preparation methods, for automation and standardization, and for multiplex analyses to preserve the breath matrix and facilitating analytical throughput. Measure exhaled constituents (e.g. CO2, acetone, isoprene) as markers of human presence or mitigate such contaminants in enclosed environments. Human and animal in vivo studies: decoding the ''breath exposome'', implementing exposure and intervention studies, performing cross-sectional and case-control research, assaying immune and inflammatory response, and testing mammalian host response to infections and exogenous exposures to develop information directly applicable to systems biology. Studying inhalation toxicology; inhaled breath as a source of internal dose; resultant blood, breath and urinary biomarkers linked to inhalation pathway. Cellular and molecular level in vitro studies. Clinical, pharmacological and forensic applications. Mathematical, statistical and graphical data interpretation.
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