{"title":"<b>COX1 gene as a promising tool for barcoding different species of <i>Euplotes</i> (Ciliophora: Euplotidae)</b>.","authors":"Mikhail M Tribun, Natalia A Lebedeva","doi":"10.11646/zootaxa.5837.2.9","DOIUrl":null,"url":null,"abstract":"<p><p><i>Euplotes</i> Ehrenberg, 1830 is a highly diversified genus of ciliates comprising over 160 species. Their identification is problematic at both the morphological and the molecular level. Here, we used an integrative approach to describe four <i>Euplotes</i> species: <i>E. vannus</i>, <i>E. aediculatus</i>, <i>E. sinicus</i> and <i>E. octocarinatus</i>. We obtained 9 new sequences: 4 sequences of the nuclear small-subunit ribosomal RNA gene and 5 sequences of the mitochondrial cytochrome <i>c</i> oxidase 1 (COX1) gene. For two of these species (<i>E. octocarinatus</i> and <i>E. sinicus)</i> the COX1 gene sequences were obtained for the first time. The topology of phylogenetic trees based on the two marker genes was the same for most of the clades. The COX1 gene showed a good resolution at the intraspecific level. This finding indicates that the COX1 gene is a promising tool for barcoding different species of <i>Euplotes</i>.</p>","PeriodicalId":24072,"journal":{"name":"Zootaxa","volume":"5837 2","pages":"365-378"},"PeriodicalIF":0.9000,"publicationDate":"2026-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zootaxa","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.11646/zootaxa.5837.2.9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ZOOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Euplotes Ehrenberg, 1830 is a highly diversified genus of ciliates comprising over 160 species. Their identification is problematic at both the morphological and the molecular level. Here, we used an integrative approach to describe four Euplotes species: E. vannus, E. aediculatus, E. sinicus and E. octocarinatus. We obtained 9 new sequences: 4 sequences of the nuclear small-subunit ribosomal RNA gene and 5 sequences of the mitochondrial cytochrome c oxidase 1 (COX1) gene. For two of these species (E. octocarinatus and E. sinicus) the COX1 gene sequences were obtained for the first time. The topology of phylogenetic trees based on the two marker genes was the same for most of the clades. The COX1 gene showed a good resolution at the intraspecific level. This finding indicates that the COX1 gene is a promising tool for barcoding different species of Euplotes.
期刊介绍：
Zootaxa is a peer-reviewed international journal for rapid publication of high quality papers on any aspect of systematic zoology, with a preference for large taxonomic works such as monographs and revisions. Zootaxa considers papers on all animal taxa, both living and fossil, and especially encourages descriptions of new taxa. All types of taxonomic papers are considered, including theories and methods of systematics and phylogeny, taxonomic monographs, revisions and reviews, catalogues/checklists, biographies and bibliographies, identification guides, analysis of characters, phylogenetic relationships and zoogeographical patterns of distribution, descriptions of taxa, and nomenclature. Open access publishing option is strongly encouraged for authors with research grants and other funds. For those without grants/funds, all accepted manuscripts will be published but access is secured for subscribers only.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
