Comparative analysis of disk diffusion and microscan versus broth microdilution methods for determining antimicrobial susceptibility of cefepime and piperacillin-tazobactam in Escherichia coli isolates carrying bla_OXA-1.

IF 4.2 2区医学 Q2 MICROBIOLOGY

Antimicrobial Agents and Chemotherapy Pub Date : 2026-08-05 Epub Date: 2026-06-22 DOI:10.1128/aac.00300-26

Marta Carretero-Ledesma, Carlos Saúco-Carballo, Ana Velázquez Escudero, Inmaculada López-Hernández, Inés Portillo-Calderón, Lorena López-Cerero, José Manuel Rodríguez-Martínez, Jesús Rodríguez Baño, Álvaro Pascual, Fernando Docobo-Pérez

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引用次数: 0

Abstract

Routine susceptibility testing often misses resistance in bla_OXA-1-producing E. coli, risking therapeutic failure. Among 165 clinical isolates from the Spanish multicenter cohort, MicroScan and disk diffusion versus reference broth microdilution yielded suboptimal categorical agreement (73.9%-86.7%) for piperacillin-tazobactam (PTZ) and cefepime (FEP). MicroScan PTZ very major errors (30%) indicate resistance underestimation; disk diffusion PTZ major errors (23%) and both methods FEP major errors (8.3%-13.8%) indicate resistance overestimation. These errors justify cautious interpretation of routine susceptibility results.

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纸片扩散法和微扫描法与肉汤微稀释法测定头孢吡肟和哌拉西林-他唑巴坦对携带blaOXA-1的大肠埃希菌的药敏比较分析。

常规的药敏试验经常遗漏产生blaoxa -1的大肠杆菌的耐药性，有治疗失败的风险。在来自西班牙多中心队列的165株临床分离株中，MicroScan和磁盘扩散与参考肉汤微量稀释对哌拉西林-他唑巴坦（PTZ）和头孢吡肟（FEP）的分类一致性不理想（73.9%-86.7%）。MicroScan PTZ非常大的误差（30%）表明电阻低估；圆盘扩散PTZ主要误差（23%）和两种方法FEP主要误差（8.3%-13.8%）表明电阻高估。这些错误证明了对常规易感性结果的谨慎解释。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antimicrobial Agents and Chemotherapy 医学-微生物学

CiteScore

10.00

自引率

8.20%

发文量

762

审稿时长

3 months

期刊介绍： Antimicrobial Agents and Chemotherapy (AAC) features interdisciplinary studies that build our understanding of the underlying mechanisms and therapeutic applications of antimicrobial and antiparasitic agents and chemotherapy.