Restoration of Gut Microbiota Dysbiosis by a Probiotic Lactobacillus paragasseri OLL2716 in Patients Being Treated with Low-Dose Aspirin and Acid-Suppressive Agents.

Abstract

Introduction: Low-dose aspirin (LDA)-associated small intestinal injury has been suggested to be associated with gut microbiota dysbiosis. The probiotic Lactobacillus paragasseri OLL2716 (LG21) mitigates LDA-induced injury; however, its mechanisms of action on gut microbiota remain unclear.

Methods: Participants were assigned to the control, acid-suppressive agents (ASAs)-, and ASAs+LDA-treated groups. All subjects consumed yogurt containing LG21 twice daily for 6 weeks. Ileal lavage and fecal microbiota were analyzed once (at the baseline) and twice (at the baseline and post-intervention), respectively.

Results: In ileal lavage at the baseline, the family Enterobacteriaceae showed a higher abundance in the ASAs+LDA group than in the control and ASAs groups. A tendency toward increased Enterobacteriaceae abundance was also observed in the feces of the ASAs group; however, it was completely restored to the levels comparable to the control group after LG21 treatment. At the baseline in the fecal microbiota, beta diversity differed significantly between the control and ASAs groups and between the control and ASAs+LDA groups, whereas no significant differences were observed among the groups after LG21 intervention. Increased Streptococcaceae and Bacteroidaceae abundances in the ASAs-treated groups at the baseline were also restored after LG21 intervention.

Conclusion: Increased Enterobacteriaceae abundance was found in both ileal lavage of the ASAs+LDA group and feces of the ASAs group. This increase in the abundance of potentially pathogenic bacteria was restored by a probiotic LG21 strain. These findings suggest that LG21 may modulate gut microbiota, particularly Enterobacteriaceae, which could be associated with its beneficial effects on LDA-induced small bowel injury.