Molecular features of external Auditory Canal cholesteatoma by microbial metagenomic sequencing

IF 3.6 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Genomics Pub Date : 2026-07-01 Epub Date: 2026-06-22 DOI:10.1016/j.ygeno.2026.111282

Guanwen He, Xiuqin Guo, Wenxian Lu, Yachun Zou, Jiayi Zheng, Xinxin Han, Yiyun Hong, Rifu Wei

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引用次数: 0

Abstract

•
4377 core genes identified, defining conserved microbial signatures in EACC.
•
Dominant taxa: Firmicutes (42.1%), Staphylococcus (32.4%), linked to pathogenesis.
•
Multidrug resistance genes (blaTEM, mecA) detected, guiding targeted therapy.
•
Functional pathways (carbohydrate metabolism) link microbiota to tissue destruction.
•
Microbial network analysis reveals key nodes in community stability.

Objective

External auditory canal cholesteatoma (EACC), a rare destructive benign lesion, causes significant hearing loss, recurrent infections, and impaired quality of life. We characterized its microbial profiles to explore associations with disease progression.

Methods

Cholesteatoma tissues from surgically treated EACC patients (2021−2022) underwent metagenomic sequencing (Illumina MiSeq). Taxonomic composition, functional genes, and antimicrobial resistance (AMR) profiles were systematically analyzed.

Results

We identified 4377 core genes revealing abundance correlations. Dominant taxa included Firmicutes (42.1%), Proteobacteria (28.6%), and Actinobacteria (19.3%), with enriched Staphylococcus (32.4%) and Corynebacterium (21.7%). Hierarchical clustering and PCA/NMDS confirmed significant taxonomic divergence. AMR profiling detected multidrug-resistant genotypes (e.g., blaTEM, mecA).

Conclusion

This study defines EACC's microbial complexity and its pathogenic role, advocating microbiome-targeted strategies to mitigate infections.

查看原文本刊更多论文

外耳道胆脂瘤微生物宏基因组测序的分子特征。

目的：外耳道胆脂瘤（EACC）是一种罕见的破坏性良性病变，可导致严重的听力损失，反复感染和生活质量下降。我们对其微生物谱进行了表征，以探索与疾病进展的关系。方法：对手术治疗的EACC患者（2021-2022）的胆脂瘤组织进行宏基因组测序（Illumina MiSeq）。系统分析了其分类组成、功能基因和耐药谱。结果：共鉴定出4377个具有丰度相关性的核心基因。优势菌群为厚壁菌门（42.1%）、变形菌门（28.6%）和放线菌门（19.3%），葡萄球菌（32.4%）和棒状杆菌（21.7%）富集。等级聚类和PCA/NMDS证实了显著的分类差异。抗菌素耐药性分析检测到多重耐药基因型（如blaTEM、mecA）。结论：本研究明确了EACC的微生物复杂性及其致病作用，提出了以微生物组为目标的减轻感染策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genomics 生物-生物工程与应用微生物

CiteScore

9.60

自引率

2.30%

发文量

260

审稿时长

60 days

期刊介绍： Genomics is a forum for describing the development of genome-scale technologies and their application to all areas of biological investigation. As a journal that has evolved with the field that carries its name, Genomics focuses on the development and application of cutting-edge methods, addressing fundamental questions with potential interest to a wide audience. Our aim is to publish the highest quality research and to provide authors with rapid, fair and accurate review and publication of manuscripts falling within our scope.