Draft genome sequence of Staphylococcus saprophyticus strain 3M isolated from milk of cows with subclinical mastitis in Uganda.

IF 2.5 Q3 GENETICS & HEREDITY

BMC genomic data Pub Date : 2026-06-04 DOI:10.1186/s12863-026-01447-1

Reagan Muhwezi, G R Neel, Emmanuel Eilu, Vidya Sankarapandian, Ambrose Shabohurira, Musoba Abubakar, Theophilus Pius, Priscilla Peter Dilli, Naheem Adekilekun Tijani, Danladi Makeri

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Abstract

Objective: This study presents the draft genome sequence of Staphylococcus saprophyticus strain 3M isolated from a cow with subclinical mastitis (SCM) in Kiruhura district, Uganda and explores the isolates resistance and virulence potential.

Data description: The assembled genome size was 2,791,445 bp with a 33% GC, 13 Contigs, Contig N50 2,589,240, Contig L50 1 and 2,791,440x Genome coverage. Check M analysis revealed 68.25% completeness. Average Nucleotide Identity (ANI) analysis revealed Staphylococcus saprophyticus subsp. saprophyticus ATCC 15,305 = NCTC 7292(GCA_006094355.1) as the closest type strain with an ANI of 99.52%, 87.49% assembly coverage, 93.95% type assembly coverage, confirming species-level identity and close relatedness. Screening with the Comprehensive Antibiotic Resistance Database (CARD) and the Virulence Factor Database (VFDB) revealed several genes conferring resistance to different classes of antibiotics: antibiotic tet (K)- Tetracyclines, norA, YkkCD, ANT (6)-I, str, and S12p- Aminoglycosides. And also, Virulence factors included: Agr- biofilm and toxins release, TcaR- Protein A, MprF- resist the host's innate immune system (specifically, antimicrobial peptides). Other genome characteristics are presented in Table 1 below. Table 1 Data availability, genome statistics and features of strain 3M Parameter Value Genes 2,885 CDSs 2,801 rRNAs 7, 6, 6 (5 S, 16 S, 23 S) tRNAs 61 ncRNAs 4.

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从乌干达亚临床乳腺炎奶牛的牛奶中分离的腐生葡萄球菌3M菌株的基因组序列草图。

目的：研究从乌干达基鲁胡拉地区一头亚临床乳腺炎（SCM）奶牛中分离的腐生葡萄球菌3M株的基因组序列草图，并探讨该菌株的耐药性和毒力潜力。数据描述：组装的基因组大小为2,791,445 bp， GC为33%，13个Contigs， Contig N50, 2,589,240， Contig L50 1和2,791,440倍基因组覆盖率。检验M分析的完备性为68.25%。平均核苷酸鉴定（ANI）分析显示为腐生葡萄球菌亚种。腐生菌ATCC 15305 = NCTC 7292（GCA_006094355.1）是最接近的类型菌株，ANI为99.52%，组合覆盖率为87.49%，类型组合覆盖率为93.95%，证实了物种水平上的同一性和亲缘关系。通过综合抗生素耐药性数据库（CARD）和毒力因子数据库（VFDB）进行筛选，发现了几种对不同类别抗生素具有耐药性的基因：抗生素tet (K)-四环素、norA、YkkCD、ANT(6)- 1、str和S12p-氨基糖苷类。此外，毒力因子包括：Agr-生物膜和毒素释放，TcaR-蛋白A， MprF-抵抗宿主先天免疫系统（特别是抗菌肽）。其他基因组特征如下表1所示。表1菌株3mparametervaluegenes2,885 cdss2,801rrnas7,6,6 (5 S, 16 S, 23 S)tRNAs61ncRNAs4的数据可用性、基因组统计及特征

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BMC genomic data

CiteScore

4.90

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0.00%

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