{"title":"Neutrophilic asthma: advances in experimental models from pathogenesis to therapeutic translation.","authors":"Jialin Yao, Jingjing Lu, Jing Gao, Ximing Liao, Di Wu, Muyun Wang, Qiang Li, Wei Gao","doi":"10.1186/s10020-026-01507-3","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Neutrophilic asthma (NA) is a severe and therapy-resistant asthma endotype marked by persistent airway inflammation, neutrophil infiltration, and limited response to glucocorticoids. The development of targeted therapies has been hampered by insufficient mechanistic insight and the lack of physiologically relevant experimental models.</p><p><strong>Main body: </strong>This review comprehensively synthesizes current in vivo and in vitro models for NA, discussing their methodologies, mechanistic fidelity, and translational potential. Murine systems provide flexible genetic and induction platforms; rat models reliably replicate steroid resistance; and equine models spontaneously mimic chronic human disease. Advanced in vitro approaches including air-liquid interface cultures, patient-derived organoids and lung-on-a-chip devices, enable precise study of epithelial barrier dysfunction, immune crosstalk, and personalized drug responses.</p><p><strong>Conclusion: </strong>By critically evaluating these tools, our review establishes a foundation for model standardization and underscores their collective role in elucidating NA pathogenesis and advancing precision medicine.</p>","PeriodicalId":18813,"journal":{"name":"Molecular Medicine","volume":" ","pages":""},"PeriodicalIF":8.3000,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13436277/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s10020-026-01507-3","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Neutrophilic asthma (NA) is a severe and therapy-resistant asthma endotype marked by persistent airway inflammation, neutrophil infiltration, and limited response to glucocorticoids. The development of targeted therapies has been hampered by insufficient mechanistic insight and the lack of physiologically relevant experimental models.
Main body: This review comprehensively synthesizes current in vivo and in vitro models for NA, discussing their methodologies, mechanistic fidelity, and translational potential. Murine systems provide flexible genetic and induction platforms; rat models reliably replicate steroid resistance; and equine models spontaneously mimic chronic human disease. Advanced in vitro approaches including air-liquid interface cultures, patient-derived organoids and lung-on-a-chip devices, enable precise study of epithelial barrier dysfunction, immune crosstalk, and personalized drug responses.
Conclusion: By critically evaluating these tools, our review establishes a foundation for model standardization and underscores their collective role in elucidating NA pathogenesis and advancing precision medicine.
期刊介绍：
Molecular Medicine is an open access journal that focuses on publishing recent findings related to disease pathogenesis at the molecular or physiological level. These insights can potentially contribute to the development of specific tools for disease diagnosis, treatment, or prevention. The journal considers manuscripts that present material pertinent to the genetic, molecular, or cellular underpinnings of critical physiological or disease processes. Submissions to Molecular Medicine are expected to elucidate the broader implications of the research findings for human disease and medicine in a manner that is accessible to a wide audience.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
