[The application of breast cancer organoids in drug screening and gene function research].

中华外科杂志 Pub Date : 2026-07-01 DOI:10.3760/cma.j.cn112139-20250707-00334

Y Q Zhang, S H Wu, J W Fang, L Q Zhang, B Xiao, L H Li

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引用次数: 0

Abstract

Organoids refer to cell clusters with certain spatial structures formed by human adult stem cells or pluripotent stem cells through three-dimensional culture in vitro, which can be stably passaged. Organoids can simulate real organs in both structure and function, and to the greatest extent reproduce the structure and function of living organs or tissues. They have three-dimensional structural features, retain cellular heterogeneity, and can self-renew and self-organize. In the field of breast cancer research, breast cancer organoid models can faithfully reproduce the heterogeneity, tissue structure, and drug response characteristics of patients' tumors, providing a powerful platform for in-depth understanding of breast cancer biology and achieving individualized treatment. Currently, significant progress has been made in drug sensitivity screening and drug resistance mechanism research using breast cancer organoids, and they have shown unique advantages in elucidating the functions of key genes. This review will introduce organoid technology, especially the application of breast cancer organoids in drug screening and gene function research, and look forward to their application prospects in promoting the development of precision medicine for breast cancer.

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乳腺癌类器官在药物筛选和基因功能研究中的应用

类器官是指人成体干细胞或多能干细胞通过体外三维培养形成具有一定空间结构的细胞团，能够稳定传代。类器官可以在结构和功能上模拟真实器官，最大程度地再现活体器官或组织的结构和功能。它们具有三维结构特征，保持细胞异质性，具有自我更新和自我组织能力。在乳腺癌研究领域，乳腺癌类器官模型能够真实再现患者肿瘤的异质性、组织结构和药物反应特征，为深入了解乳腺癌生物学，实现个体化治疗提供了有力的平台。目前，利用乳腺癌类器官进行药物敏感性筛选和耐药机制研究取得了重大进展，在阐明关键基因功能方面显示出独特的优势。本文将介绍类器官技术，特别是乳腺癌类器官在药物筛选和基因功能研究中的应用，并展望其在促进乳腺癌精准医学发展方面的应用前景。

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来源期刊

中华外科杂志

CiteScore

0.80

自引率

0.00%

发文量

20861

期刊介绍： Chinese Journal of Surgery|Chin J Surg (monthly) is a high-level medical science and technology journal approved by the General Administration of Press and Publication of the People's Republic of China, under the supervision of the China Association for Science and Technology, and organised by the Chinese Medical Association for domestic and international public circulation. It was founded in January 1951, and is published on the basis of the Journal of Chinese Surgery. The Journal is aimed at senior and intermediate surgeons and related researchers, mainly reporting the leading scientific research results and clinical experience in the field of surgery, as well as the basic theoretical research that has a guiding effect on the clinical work of surgery. Chinese Journal of Surgery|Chin J Surg is committed to reflecting the major research progress in the field of surgery in China and promoting academic exchanges at home and abroad. The main columns include thesis, meta-analysis, review, expert forum, synthesis, case report, diagnosis and treatment experience, technical exchange, clinical case discussion, academic controversy, and special lectures, etc. The journal has been accepted by the National Academy of Medicine of the United States. The journal has been included in many famous databases at home and abroad, such as the Biomedical Analysis and Online Retrieval System (MEDLINE) of the U.S. National Library of Medicine.