The curious case of stomach diverticula in Epiphanes clavulata (Rotifera: Monogononta).

Abstract

Rotifers are aquatic micrometazoans with a variety of feeding styles and dietary preferences. The morphology of their digestive tracts is well known, but there are few details on the structure of any organs. Here we use transmission electron microscopy and fluorescence microscopy to investigate the stomach of Epiphanes clavulata, which is unusual in its possession of 6 diverticula that are absent in other taxa. We focus on this region to determine whether these diverticula are exocrine and potentially homologous to gastric glands, which are the main digestive organs of rotifers, but absent in this species. Results reveal that the stomach is cellular and that all cells are ciliated, nucleated, and contain abundant phagosomes and lipid droplets. The stomach appears to play 2 major roles: intracellular digestion as evidenced by the presence of caveolae and phagosomes and lipid biogenesis and storage as evidenced by accumulation of large lipid droplets. The 6 diverticula are syncytial extensions of individual stomach cells and contain 1 to several nuclei, many mitochondria, autophagic vesicles, ribosomes, and inclusion bodies. The rough endoplasmic reticulum is abundant and associated with small electron-dense droplets enclosed in a phospholipid monolayer, suggesting they contain lipids; however, the absence of discernable staining with a neutral lipid dye confounds a more specific identification of their contents. There is no evidence to suggest the diverticula are homologous to gastric glands nor do they play an obvious role in digestion; instead, they may function as a secondary site of lipid biogenesis and storage.