hsa_circ_0005963 downregulation suppresses NSCLC malignant progression by miR-506-3p/SOX9-mediated stemness regulation.

IF 3.1 4区医学 Q2 GENETICS & HEREDITY

Epigenomics Pub Date : 2026-05-01 Epub Date: 2026-05-21 DOI:10.1080/17501911.2026.2654577

Xuechao Zhou, Yu Yao, Huaiyin Ding

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引用次数: 0

Abstract

Purpose: This study investigated the role and regulatory mechanisms underlying hsa_circ_0005963 in NSCLC.

Methods: FISH and RT-qPCR were used to assess hsa_circ_0005963 expression in NSCLC cells and tissues. Dual-luciferase reporter assay was employed to characterize hsa_circ_0005963 downstream targets. Transwell migration and EdU incorporation were performed to detect NSCLC cell proliferation and migration. A mouse tumor xenograft model was constructed to assess hsa_circ_0005963 functions in NSCLC metastasis and progressions.

Results: hsa_circ_0005963 expression was upregulated in NSCLC tissues and cells. High hsa_circ_0005963 expression indicates poor prognosis, including overall survival. hsa_circ_0005963 silencing inhibited NSCLC proliferation and migration. Experiments confirmed that hsa_circ_0005963 downregulation suppresses A549 tumor invasions. A dual-luciferase reporter assay identified SOX9 and microRNA as hsa_circ_0005963 downstream targets, which was validated by RT-qPCR. SOX9 overexpression or miR-506-3p downregulation reversed the hsa_circ_0005963 silencing inhibitory effects upon proliferation and migrations. SOX9 overexpression reversed miR-506-3p effects to inhibit proliferation and migration in both cell lines.

Conclusion: hsa_circ_0005963 promotes NSCLC development by sponging miR-506-3p to enhance SOX9 expression.

查看原文本刊更多论文

hsa_circ_0005963下调通过miR-506-3p/ sox9介导的干性调控抑制NSCLC恶性进展。

目的：本研究探讨hsa_circ_0005963在非小细胞肺癌中的作用及其调控机制。方法：采用FISH和RT-qPCR检测hsa_circ_0005963在NSCLC细胞和组织中的表达。采用双荧光素酶报告基因法对hsa_circ_0005963下游靶点进行表征。Transwell迁移和EdU掺入检测NSCLC细胞的增殖和迁移。构建小鼠肿瘤异种移植模型，评估hsa_circ_0005963在非小细胞肺癌转移和进展中的功能。结果：hsa_circ_0005963在NSCLC组织和细胞中表达上调。hsa_circ_0005963高表达提示预后差，包括总生存期。hsa_circ_0005963沉默抑制NSCLC的增殖和迁移。实验证实hsa_circ_0005963下调可抑制A549的肿瘤侵袭。双荧光素酶报告基因检测鉴定出SOX9和microRNA是hsa_circ_0005963的下游靶标，并通过RT-qPCR验证。SOX9过表达或miR-506-3p下调逆转了hsa_circ_0005963沉默对增殖和迁移的抑制作用。SOX9过表达逆转了miR-506-3p的作用，抑制了两种细胞系的增殖和迁移。结论：hsa_circ_0005963通过海绵miR-506-3p增强SOX9表达促进NSCLC的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Epigenomics GENETICS & HEREDITY-

CiteScore

5.80

自引率

2.60%

发文量

审稿时长

>12 weeks

期刊介绍： Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.