Analysis of the trends in in vitro studies of the Latin America-Comet assay (LA-COMET) Group

IF 2.8 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2026-05-01 Epub Date: 2026-05-08 DOI:10.1016/j.mrgentox.2026.503939

Mahara Valverde , Ana Rosa Flores-Márquez , Sandra Gómez-Arroyo , Laura Lafon-Hughes , Grethel León-Mejía , Diana M. Narváez , Sonia Soloneski , Noemi Tirado , Juliana Da Silva , Guillermo Espinosa-Reyes , Deidamia Franco , Ana Leticia García , Patricia Mussali-Galante , Elda Pacheco-Pantoja , Gisela Poletta , Celeste Ruiz de Arcaute , María Fernanda Simoniello , Efraín Tovar-Sánchez , Leticia Yáñez- Estrada , Emilio Rojas

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引用次数: 0

Abstract

On a global scale, the versatility and high sensitivity of the comet assay have promoted its use from different perspectives to determine genotoxic damage. With this in mind and through the formation of a Latin American group denominated "LA-COMET", a review of the manuscripts published by the group is proposed from the perspective of in vitro studies, using the alkaline comet assay. To facilitate the analysis of the 81 manuscripts published between 1996 and 2022, they were classified into four categories based on the type of agents evaluated by the assay: 1) environmental pollutants; 2) pesticides and their mixtures; 3) radiation and drugs; and 4) natural products. The objective of this review was to describe the strengths and versatility of in vitro studies reflected by cellular models, putative DNA damaging agents, and the use of specific enzymes to identify particular DNA lesions. In addition, an in vitro QS (quality score) was established, taking into consideration the data provided in the publications regarding the cellular model, the method of disaggregation and the cell suspension obtention. Other considerations included the clarity of the description of the treatments, the use of negative and positive reference controls, and the use of specific enzymes and/or the functional evaluation of DNA repair. The analysis demonstrated that the publications of the LA-COMET group of in vitro models exhibited a high score with respect to the important parameters established in the OECD agreements.

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拉丁美洲-彗星试验（LA-COMET）组体外研究趋势分析

在全球范围内，彗星试验的多功能性和高灵敏度从不同的角度促进了它的使用，以确定基因毒性损伤。考虑到这一点，并通过成立一个拉丁美洲小组，命名为“LA-COMET”，从体外研究的角度，使用碱性彗星试验，对该小组发表的手稿进行审查。为了便于对1996年至2022年间发表的81份手稿进行分析，根据检测评估的试剂类型将它们分为四类：1)环境污染物；（二）农药及其混合物；3)辐射和药物；4)天然产物。本综述的目的是描述细胞模型、假定的DNA损伤剂和使用特定酶来识别特定DNA损伤所反映的体外研究的优势和多功能性。此外，结合文献中关于细胞模型、解聚方法和细胞悬液观察的数据，建立体外质量评分（QS）。其他考虑因素包括治疗描述的清晰度，阴性和阳性对照的使用，特定酶的使用和/或DNA修复的功能评估。分析表明，LA-COMET组体外模型的出版物在经合组织协议中建立的重要参数方面表现出很高的得分。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.