AS-IV's protective potential against inflammatory injury via miR-320b/PTEN axis in acute ischaemic stroke.

IF 1.5 4区医学 Q4 PHARMACOLOGY & PHARMACY

Xenobiotica Pub Date : 2026-06-01 Epub Date: 2026-05-27 DOI:10.1080/00498254.2026.2672140

Xiaofen Yu, Yunjing Wang, Ting Yang, Jiancheng Lou

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引用次数: 0

Abstract

Astragaloside IV (AS-IV) represented a promising therapeutic candidate for acute ischaemic stroke. However, its definitive molecular targets and mode of action await full elucidation.The study investigated the protective efficacy of AS-IV and delineated its underlying mechanism in regulating human brain microvascular endothelial cells (BMECs) following oxygen-glucose deprivation/reoxygenation (OGD/R) conditions.Human BMECs were exposed to OGD conditions for 4 h and AS-IV (0-100 µM) was added to the medium immediately for reoxygenating treatment. MiR-320b levels were manipulated 24 h after OGD/R by transient transfection with its inhibitor or mimic. Cell viability and apoptosis were assessed by corresponding kits. The expression level of miR-320b or PTEN was achieved by RT-qPCR. Pro-inflammatory cytokines were detected by ELISA.AS-IV rescued cells under OGD/R condition by enhancing cell viability, curbing apoptosis and pro-inflammatory signalling. This aligned with the pro-survival signature of miR-320b upregulation in OGD/R cells. Conversely, miR-320b knockdown abrogated the AS-IV efficacy. RIP and dual-luciferase reporter assay confirmed PTEN as the direct downstream target of miR-320b.AS-IV protected cells under OGD/R conditions by improving viability and inhibiting inflammation as well as apoptosis via miR-320b/PTEN axis.

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AS-IV通过miR-320b/PTEN轴在急性缺血性卒中中抗炎症损伤的保护潜力。

1. 黄芪甲苷（AS-IV）是一种很有前途的治疗急性缺血性卒中的候选药物。然而，其明确的分子靶点和作用方式有待于充分阐明。本研究探讨了AS-IV在氧-葡萄糖剥夺/再氧化（OGD/R）条件下对人脑微血管内皮细胞（BMECs）的保护作用，并阐述了其调节的潜在机制。将人bmec暴露于OGD条件下4小时，并立即向培养基中加入AS-IV（0-100µM）进行再氧化处理。通过短暂转染其抑制剂或模拟物，在OGD/R后24小时控制MiR-320b水平。采用相应试剂盒检测细胞活力和凋亡。RT-qPCR检测miR-320b或PTEN的表达水平。elisa法检测促炎细胞因子。AS-IV通过增强细胞活力、抑制细胞凋亡和促炎信号传导来拯救OGD/R条件下的细胞。这与OGD/R细胞中miR-320b上调的促生存特征一致。相反，miR-320b敲低会消除AS-IV的疗效。RIP和双荧光素酶报告基因实验证实PTEN是miR-320b.5的直接下游靶点。在OGD/R条件下，as - iv通过miR-320b/PTEN轴提高细胞活力，抑制炎症和凋亡，从而保护细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Xenobiotica 医学-毒理学

CiteScore

3.80

自引率

5.60%

发文量

审稿时长

2 months

期刊介绍： Xenobiotica covers seven main areas, including:General Xenobiochemistry, including in vitro studies concerned with the metabolism, disposition and excretion of drugs, and other xenobiotics, as well as the structure, function and regulation of associated enzymesClinical Pharmacokinetics and Metabolism, covering the pharmacokinetics and absorption, distribution, metabolism and excretion of drugs and other xenobiotics in manAnimal Pharmacokinetics and Metabolism, covering the pharmacokinetics, and absorption, distribution, metabolism and excretion of drugs and other xenobiotics in animalsPharmacogenetics, defined as the identification and functional characterisation of polymorphic genes that encode xenobiotic metabolising enzymes and transporters that may result in altered enzymatic, cellular and clinical responses to xenobioticsMolecular Toxicology, concerning the mechanisms of toxicity and the study of toxicology of xenobiotics at the molecular levelXenobiotic Transporters, concerned with all aspects of the carrier proteins involved in the movement of xenobiotics into and out of cells, and their impact on pharmacokinetic behaviour in animals and manTopics in Xenobiochemistry, in the form of reviews and commentaries are primarily intended to be a critical analysis of the issue, wherein the author offers opinions on the relevance of data or of a particular experimental approach or methodology