Identification, Localization, and Expression Analysis of 5-HT6 Receptor, and Primary Role in Sepiella japonica, Based on Sex and Life Stage

IF 2.3 3区生物学 Q2 BIODIVERSITY CONSERVATION

Diversity-Basel Pub Date : 2025-01-30 DOI:10.3390/d17020104

Wenbo Cui, Prisca John Issangya, Shuang Li, Xu Zhou, Libing Zheng, Chang‐Feng Chi

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引用次数: 0

Abstract

5-Hydroxytryptamine (5-HT) plays a vital role in the reproductive process of vertebrates and is also present in many invertebrates. The cDNA of the Sepiella japonica 5-HT6 receptor (Sj5-HT6r) was first cloned by RACE (Rapid Amplification of cDNA Ends). The length was 1450 bp, and the predicted open reading frame (ORF) was 1116 bp, which encoded 371 amino acids. Sequence characteristics analysis showed that Sj5-HT6r shares a high degree of identity with 5-HT6r from other cephalopods and forms a sister branch to bivalves. Subcellular localization showed that Sj5-HT6r protein was localized on the HEK293T cell membrane surface. Quantitative Real-time PCR (qPCR) analysis demonstrated that Sj5-HT6r was highly expressed in reproductive organs of both sexes. In particular, transcripts with significant expression were observed at stage III of female gonadal development in tissues of the ovary and nidamental gland, and at stage IV in tissues of the accessory nidamental gland. In situ hybridization (ISH) experiment results indicated that Sj5-HT6r mRNA was primarily distributed in all regions of the optic lobes except the plexiform zone. These results may provide a basis for the future exploration of the reproductive regulation of 5-HT and 5-HT6 receptors in S. japonica.

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基于性别和生命阶段的5-HT6受体鉴定、定位、表达分析及其在日本海蛾中的主要作用

5-羟色胺（5-HT）在脊椎动物的生殖过程中起着至关重要的作用，也存在于许多无脊椎动物中。采用RACE （Rapid Amplification of cDNA Ends）技术首次克隆了日本血吸虫5-HT6受体（Sj5-HT6r）的cDNA。全长1450 bp，预测开放阅读框（ORF）长度为1116 bp，编码371个氨基酸。序列特征分析表明，Sj5-HT6r与其他头足类动物的5-HT6r具有高度的同源性，是双壳类动物的姐妹分支。亚细胞定位表明，Sj5-HT6r蛋白定位于HEK293T细胞膜表面。实时荧光定量PCR （qPCR）结果显示，Sj5-HT6r在两性生殖器官中均有高表达。特别是在雌性性腺发育的第三阶段，在卵巢和内腺组织中，以及在副内腺组织的第四阶段，有显著表达的转录本。原位杂交（ISH）实验结果表明，Sj5-HT6r mRNA主要分布在视叶丛状区以外的所有区域。这些结果可为进一步探索5-HT和5-HT6受体在粳稻中的生殖调控机制提供基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diversity-Basel Environmental Science-Ecological Modeling

CiteScore

3.40

自引率

12.50%

发文量

925

审稿时长

11 weeks

期刊介绍： Diversity (ISSN 1424-2818) is an international and interdisciplinary journal of science concerning diversity concept and application, diversity assessment and diversity preservation. It is focused on organismic and molecular diversity. It publishes reviews, regular research papers and short notes in the regular issues. Related news and announcements are also published. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of the papers. Full experimental details must be provided so that the results can be reproduced.