Potential mechanism of Astragalus polysaccharide increasing chemosensitivity of cervical cancer HeLa cells to carboplatin by modulating the PI3K/Akt signaling pathway

IF 0.5 4区医学 Q4 OBSTETRICS & GYNECOLOGY

European journal of gynaecological oncology Pub Date : 2023-01-01 DOI:10.22514/ejgo.2023.093

Wenzhi Liu, Lu Zhang, Minmin Yu, D Song, W Kong, T Zhang, C Han, T Liu, S Jiao, A Choudhari, P Mandave, M Deshpande, P Ranjekar, O Prakash, Y Guo, Z Zhang, Z Wang, G Liu, Y Liu, H Wang, O Bamodu, K-T Kuo, C-H Wang, W-C Huang, Ath Wu, J-T Tsai, W-C Huang, K-T Kuo, O Bamodu, Y-K Lin, C-H Wang, K-Y Lee, C Wu, Y Ke, Y Zeng, Y Zhang, H Yu, C Li, L Hong, C Liu, J Min, M Hu, W Guo, Y Tan, L Yin, Z Sun, S Shao, Chen Man, X, Q Zhai, X Hu, Xiao Yu, D, Y Shi, X Feng, L Xie, X Wang, Z Zhang, Z Wang, M Hao, T Li, Q Zhou, W Zhang, W Liu, G Li, H Zhou, Y Liu, M Luo, Z Lin, C Porta, C Paglino, A Mosca, P Xia, X-Y Xu, M Aoki, T Fujishita, T Ersahin, N Tuncbag, R Cetin-Atalay, E Lien, C Dibble, A Toker, S Noorolyai, N Shajari, E Baghbani, S Sadreddini, B Baradaran, Q Yang, W Jiang, P Hou, S Pompura, M Dominguez-Villar, Y Xie, X Shi, K Sheng, G Han, W Li, Q Zhao, J Karar, A Maity, Y Cao, Y Ruan, T Shen, X Huang, M Li, W Yu, Y Zhang, L Wang, M Du, S Sun, X He, D Luo, C Lv, S Jing, A Lv, M Zhang, W Li, P Zhao, W Gao, L Huo, A Sui, M Zhang, C Zhang, X Liu, W Li, W Gao, A Sui, Y Yang, Z Lin, P He, H Nie, Q Yao, S Zhang, L Guo, S Bai, L Zhao, X Wang, Q Tian, M Yan, H Cai, Q Tan, W Zhang

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引用次数: 0

Abstract

The current study investigated the possible mechanisms in which astragalus polysac-charide (APS) elevates the chemosensitivity of cervical cancer (CC) HeLa cells to carboplatin (CBP) by regulating the PI3K/Akt pathway. In this study, HeLa cells were assigned to the control, CBP, APS, and APS + CBP groups, and the proliferation of HeLa cells in each group was detected using the MTT (methyl thiazolyl tetrazolium) assay. The apoptosis and cycle of HeLa cells were examined using flow cytometry, and the expression levels of PI3K (phosphatidylinositol-3-kinases), Akt (Protein Kinase B), p-PI3K (phospho-phosphatidylinositol-3-kinases), and p-Akt (phospho-Akt) proteins in the PI3K/Akt pathway were determined using Western blot. MTT results revealed that the inhibitory effect was more significant within the APS + CBP groups (p < 0.01). The flow cytometry results showed that the apoptosis rate was significantly elevated in the APS + CBP groups (p < 0.01). Compared with the control group, the most prominent arrest in the G2/M phase and the arrest within the G2/M phase in the APS + CBP groups being the most significant. Western-blot results depicted that the above proteins in the APS + CBP groups revealed more significant expression changes (p < 0.01). The above analyses demonstrated that APS could increase the chemosensitivity of CC HeLa cells to CBP by modulating the PI3K/Akt pathway. In conclusions, it is reasonable to believe that the enhancing effect of APS on the sensitivity of chemotherapeutic drugs was associated with blocking the PI3K/Akt pathway, thus, enhancing.

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黄芪多糖通过调节PI3K/Akt信号通路提高宫颈癌HeLa细胞对卡铂化学敏感性的潜在机制

本研究探讨黄芪多糖（APS）通过调节PI3K/Akt通路，提高宫颈癌（CC） HeLa细胞对卡铂（CBP）的化学敏感性的可能机制。本研究将HeLa细胞分为对照组、CBP组、APS组和APS + CBP组，采用MTT（甲基噻唑四氮唑）法检测各组HeLa细胞的增殖情况。流式细胞术检测HeLa细胞的凋亡和周期，Western blot检测PI3K/Akt通路中PI3K（磷脂酰肌醇-3-激酶）、Akt（蛋白激酶B）、p-PI3K（磷脂酰肌醇-3-激酶）、p-Akt（磷酸化-Akt）蛋白的表达水平。MTT结果显示，APS + CBP组的抑制作用更为显著（p < 0.01）。流式细胞术结果显示，APS + CBP组细胞凋亡率显著升高（p < 0.01）。与对照组比较，APS + CBP组G2/M期骤停最显著，G2/M期骤停最显著。Western-blot结果显示，上述蛋白在APS + CBP组中表达变化更为显著（p < 0.01）。以上分析表明，APS可通过调节PI3K/Akt通路，提高CC HeLa细胞对CBP的化学敏感性。综上所述，我们有理由认为APS对化疗药物敏感性的增强作用与阻断PI3K/Akt通路有关，从而增强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European journal of gynaecological oncology 医学-妇产科学

自引率

25.00%

发文量

审稿时长

1 months

期刊介绍： EJGO is dedicated to publishing editorial articles in the Distinguished Expert Series and original research papers, case reports, letters to the Editor, book reviews, and newsletters. The Journal was founded in 1980 the second gynaecologic oncology hyperspecialization Journal in the world. Its aim is the diffusion of scientific, clinical and practical progress, and knowledge in female neoplastic diseases in an interdisciplinary approach among gynaecologists, oncologists, radiotherapists, surgeons, chemotherapists, pathologists, epidemiologists, and so on.