Interfacial Mechanism of Biological Property Enhancement in Irradiated MAO Coatings by HIPIB

IF 3.4 3区材料科学 Q2 MATERIALS SCIENCE, COATINGS & FILMS

Coatings Pub Date : 2025-10-09 DOI:10.3390/coatings15101184

Yi Wei, Yujie Shao, Yongxin Jiao, Xiaoguang Han

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引用次数: 0

Abstract

Magnesium alloy micro-arc oxidation (MAO) coatings are limited in biomedical applications due to their poor corrosion resistance. High-intensity pulsed ion beam (HIPIB) treatment enhances corrosion resistance as well as biocompatibility, but the underlying mechanisms are not well understood. In this study, CCK-8 assays, flow cytometry, and ALP activity tests were employed to investigate the bioactivity of the MAO coatings, and the surface properties of the coatings were characterized by SEM observation. Compared with pristine coating, the porosity of the MAO coating decreased by 9.44%, calcium content increased by 0.23%, and surface roughness and hydrophobicity increased to 7.57 and 102.11, respectively, with HIPIB irradiation. CCK-8 assays showed that the HIPIB-modified coating significantly improved cell proliferation, with a growth rate increase to 61.29% on Day 3. Flow cytometry analysis revealed accelerated cell cycle progression, especially a faster transition from the G1 to S and G2 phases, indicative of enhanced proliferation. Increased ALP activity further indicated that the irradiated coatings promoted osteogenic differentiation. The formed remelted dense layer with an increase in Ca content and high roughness induced by HIPIB irradiation not only acts as a corrosion barrier but also promotes the adhesion and differentiation of osteoblasts, which is mainly responsible for the enhancement of biological properties.

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HIPIB增强辐照MAO涂层生物性能的界面机理

镁合金微弧氧化（MAO）涂层的耐腐蚀性较差，限制了其在生物医学领域的应用。高强度脉冲离子束（HIPIB）处理提高了耐腐蚀性和生物相容性，但其潜在机制尚不清楚。本研究采用CCK-8法、流式细胞术和ALP活性试验对MAO涂层的生物活性进行了研究，并通过扫描电镜对涂层的表面特性进行了表征。与原始涂层相比，经HIPIB辐照后的MAO涂层孔隙率降低了9.44%，钙含量提高了0.23%，表面粗糙度和疏水性分别提高到7.57和102.11。CCK-8实验显示，hipib修饰的包被显著提高了细胞的增殖能力，第3天细胞的生长速率提高到61.29%。流式细胞术分析显示细胞周期进程加快，特别是从G1期到S期和G2期的转变更快，表明增殖增强。ALP活性的增加进一步表明辐照涂层促进了成骨分化。在HIPIB照射下形成的Ca含量增加、粗糙度高的重熔致密层不仅起到了腐蚀屏障的作用，而且促进了成骨细胞的粘附和分化，这是生物性能增强的主要原因。

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来源期刊

Coatings Materials Science-Surfaces, Coatings and Films

CiteScore

5.00

自引率

11.80%

发文量

1657

审稿时长

1.4 months

期刊介绍： Coatings is an international, peer-reviewed open access journal of coatings and surface engineering. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided. There are, in addition, unique features of this journal: * manuscripts regarding research proposals and research ideas will be particularly welcomed * electronic files or software regarding the full details of the calculation and experimental procedure - if unable to be published in a normal way - can be deposited as supplementary material