Analysis of retinal ganglion cell subtypes across six different inbred mouse strains.

IF 1.4 3区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Vision Pub Date : 2026-02-20 eCollection Date: 2026-01-01

Su-Ting Lin, Fangyu Lin, Jiaxing Wang, Eldon E Geisert

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引用次数: 0

Abstract

Purpose: Retinal ganglion cells (RGCs) are the principal conduits responsible for propagating visual stimuli from the retina to visual centers in the brain. The loss of RGCs leads to visual deficits following trauma or in diseases such as glaucoma. Mouse models are consistently used to investigate root causes for RGC loss. This study quantifies the total number of RGCs and selected RGC subtypes across six strains of inbred mice used in ophthalmic research.

Methods: Six mouse strains (C57BL/6J, BALB/cByJ, 129X1/SvJ, A/J, CBA/CaJ, and CAST/EiJ) were selected to represent genetic diversity across the mouse genome. Normal retinas were immunostained for POU6F2, BRN3A, SATB2, OPN4, SMI32, and TO-PRO-3. Cells positively labeled for POU6F2, BRN3A, and SATB2 were quantified using an automated deep learning tool, RGCode. Cells labeled with OPN4, SMI32, and TO-PRO-3 were quantified using the Fiji software.

Results: We found statistically significant differences in the quantity (Mean±SEM) and percentage of different RGCs across the inbred mouse strains. The total number of RGCs per retina ranged from 39,961±838 in CAST/EiJ to 53,872±1864 in 129X1/SvJ (p<0.005). Global BRN3A counts ranged from 34,572±494 in CAST/EiJ to 44,253±798 in C57BL/6J (p<0.005). SATB2 counts ranged from 9944±384 in BALB/cByJ to 15,872±1196 in CBA/CaJ (p<0.005). OPN4 density ranged from 110±7 cells/mm² in CAST/EiJ to 164±13 cells/mm² in 129X1/SvJ (p<0.05). Differences in SMI32 density were not significant across all strains, with densities ranging from 183±14 cells/mm² in A/J to 279±12 cells/mm² in C57BL/6J (not significant).

Conclusions: There is a significant variation in total RGC counts and RGC subtypes across the different mouse strains. When working with different strains of mice, it is important to consider this strain-based variation before drawing conclusions from experimental data.

本刊更多论文

六种不同近交系小鼠视网膜神经节细胞亚型分析。

目的：视网膜神经节细胞（RGCs）是负责将视觉刺激从视网膜传递到大脑视觉中枢的主要通道。RGCs的缺失导致外伤或青光眼等疾病后的视力缺陷。小鼠模型一直被用于研究RGC损失的根本原因。本研究量化了用于眼科研究的6种近交系小鼠的RGC总数和选定的RGC亚型。方法：选取6个小鼠品系（C57BL/6J、BALB/cByJ、129X1/SvJ、A/J、CBA/CaJ和CAST/EiJ）代表小鼠基因组的遗传多样性。对正常视网膜进行POU6F2、BRN3A、SATB2、OPN4、SMI32和TO-PRO-3的免疫染色。使用自动深度学习工具RGCode对POU6F2、BRN3A和SATB2阳性标记的细胞进行定量。使用Fiji软件对OPN4、SMI32和TO-PRO-3标记的细胞进行定量。结果：各近交系小鼠不同RGCs的数量（均值±SEM）和百分比差异有统计学意义。每视网膜RGCs总数在CAST/EiJ组为39,961±838至129X1/SvJ组为53,872±1864 (CAST/EiJ组为p2至129X1/SvJ组为164±13个细胞/mm2 (A/J组为p2至C57BL/6J组为279±12个细胞/mm2)（差异无统计学意义）。结论：不同小鼠品系的RGC总数和RGC亚型存在显著差异。当使用不同的小鼠品系时，在从实验数据中得出结论之前，考虑这种基于品系的差异是很重要的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Vision 生物-生化与分子生物学

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Molecular Vision is a peer-reviewed journal dedicated to the dissemination of research results in molecular biology, cell biology, and the genetics of the visual system (ocular and cortical). Molecular Vision publishes articles presenting original research that has not previously been published and comprehensive articles reviewing the current status of a particular field or topic. Submissions to Molecular Vision are subjected to rigorous peer review. Molecular Vision does NOT publish preprints. For authors, Molecular Vision provides a rapid means of communicating important results. Access to Molecular Vision is free and unrestricted, allowing the widest possible audience for your article. Digital publishing allows you to use color images freely (and without fees). Additionally, you may publish animations, sounds, or other supplementary information that clarifies or supports your article. Each of the authors of an article may also list an electronic mail address (which will be updated upon request) to give interested readers easy access to authors.