{"title":"Establishment of LPS-induced sepsis and TNF-induced SIRS.","authors":"Jianling Liu, Daichao Xu","doi":"10.1016/bs.mcb.2026.01.015","DOIUrl":null,"url":null,"abstract":"<p><p>Programmed cell death (PCD) serves as a fundamental biological mechanism essential for maintaining cellular homeostasis and orchestrating physiological processes, while its dysregulation constitutes a hallmark of diverse pathological conditions. Substantial evidence has implicated aberrant PCD pathways in the pathogenesis of inflammatory diseases, autoimmune diseases and neurodegenerative disorders. Despite the various in vitro systems for cell death investigation, in vivo models retain significant advantages. The current research proposal specifically delineates two well-characterized in vivo models: (1) lipopolysaccharide (LPS)-induced sepsis model and (2) tumor necrosis factor-alpha (TNF-α)-mediated systemic inflammatory response syndrome (SIRS) model. These established in vivo experimental systems enable more convincing evidence for cell death research.</p>","PeriodicalId":18437,"journal":{"name":"Methods in cell biology","volume":"205 ","pages":"189-198"},"PeriodicalIF":0.0000,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Methods in cell biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/bs.mcb.2026.01.015","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2026/1/29 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Programmed cell death (PCD) serves as a fundamental biological mechanism essential for maintaining cellular homeostasis and orchestrating physiological processes, while its dysregulation constitutes a hallmark of diverse pathological conditions. Substantial evidence has implicated aberrant PCD pathways in the pathogenesis of inflammatory diseases, autoimmune diseases and neurodegenerative disorders. Despite the various in vitro systems for cell death investigation, in vivo models retain significant advantages. The current research proposal specifically delineates two well-characterized in vivo models: (1) lipopolysaccharide (LPS)-induced sepsis model and (2) tumor necrosis factor-alpha (TNF-α)-mediated systemic inflammatory response syndrome (SIRS) model. These established in vivo experimental systems enable more convincing evidence for cell death research.
期刊介绍：
For over fifty years, Methods in Cell Biology has helped researchers answer the question "What method should I use to study this cell biology problem?" Edited by leaders in the field, each thematic volume provides proven, state-of-art techniques, along with relevant historical background and theory, to aid researchers in efficient design and effective implementation of experimental methodologies. Over its many years of publication, Methods in Cell Biology has built up a deep library of biological methods to study model developmental organisms, organelles and cell systems, as well as comprehensive coverage of microscopy and other analytical approaches.
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