Longitudinal study of genome-wide DNA methylation in individuals with and without post-acute symptoms following SARS-CoV-2 infection.

IF 3.1 4区医学 Q2 GENETICS & HEREDITY

Epigenomics Pub Date : 2026-04-01 Epub Date: 2026-04-10 DOI:10.1080/17501911.2026.2656361

Jon Bohlin, Yunsung Lee, Ida Henriette Caspersen, Anna Hayman Robertson, Christian M Page, Håkon K Gjessing, Astanand Jugessur, Per Magnus, Siri Mjaaland, Lill Trogstad

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引用次数: 0

Abstract

Background: Symptoms following SARS-CoV-2 infection, referred to as Long-COVID, have been reported since the pandemic. We investigated whether COVID-19 or Long-COVID is associated with persistent genome-wide DNA methylation (DNAm) changes in whole blood using a longitudinal design.

Methods: DNAm was measured using the Illumina EPIC V2 platform (859,651 CpGs) in 297 adult participants (594 samples in total) from two Norwegian population-based cohorts, with samples collected pre-infection (2020) and during the pandemic (2023). Participants were classified as Long-COVID, COVID-19 (no persistent symptoms), or not infected.

Results: No significant DNAm differences were observed between Long-COVID and not infected at either time point (p = 0.745(FDR)) or during the pandemic specifically (p = 0.629(FDR)). Likewise, no differences were detected between COVID-19 and not infected across both time points (p = 0.883(FDR)) or during the pandemic (p = 0.287(FDR)). Sex-stratified analyses of the X chromosome revealed no significant DNAm differences for Long-COVID or COVID-19 in males (both p = 0.999(FDR)) or females (both p = 0.999(FDR)). Epigenetic age acceleration was also evaluated using DunedinPACE (DP) and PhenoAge (PA), but no significant differences were detected for Long-COVID (p = 0.695 [DP], p = 0.528 [PA]) or COVID-19 (p = 0.624 [DP], p = 0.348 [PA]).

Conclusion: No persistent epigenetic age- or DNAm based differences due to Long-COVID or SARS-CoV-2 infection were detected in our cohorts.

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在有和没有SARS-CoV-2感染后急性后症状的个体中全基因组DNA甲基化的纵向研究

背景：SARS-CoV-2感染后的症状，称为长冠状病毒，自大流行以来一直有报告。我们使用纵向设计研究了COVID-19或Long-COVID是否与全血中持续的全基因组DNA甲基化（DNAm）变化相关。方法：使用Illumina EPIC V2平台测量来自两个挪威人群队列的297名成年参与者（总共594份样本）的DNAm (859,651 CpGs)，样本收集于感染前（2020年）和大流行期间（2023年）。参与者被分为长冠、COVID-19（无持续症状）和未感染。结果：在任何时间点（p = 0.745(FDR)）或大流行期间（p = 0.629(FDR)），长冠和未感染之间均未观察到显著的DNAm差异。同样，在两个时间点（p = 0.883(FDR)）或大流行期间（p = 0.287(FDR)）， COVID-19和未感染之间没有发现差异。X染色体的性别分层分析显示，Long-COVID或COVID-19在男性（p = 0.999(FDR)）或女性（p = 0.999(FDR)）中没有显著的DNAm差异。使用DunedinPACE （DP）和PhenoAge （PA）也评估了表观遗传年龄加速，但Long-COVID （p = 0.695 [DP], p = 0.528 [PA]）和covid （p = 0.624 [DP], p = 0.348 [PA]）没有发现显著差异。结论：在我们的队列中未发现长期covid或SARS-CoV-2感染导致的持续表观遗传年龄或dna差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Epigenomics GENETICS & HEREDITY-

CiteScore

5.80

自引率

2.60%

发文量

审稿时长

>12 weeks

期刊介绍： Epigenomics provides the forum to address the rapidly progressing research developments in this ever-expanding field; to report on the major challenges ahead and critical advances that are propelling the science forward. The journal delivers this information in concise, at-a-glance article formats – invaluable to a time constrained community. Substantial developments in our current knowledge and understanding of genomics and epigenetics are constantly being made, yet this field is still in its infancy. Epigenomics provides a critical overview of the latest and most significant advances as they unfold and explores their potential application in the clinical setting.