Exploration of the diagnostic and therapeutic potential of the nucleocytoplasmic shuttling protein TMUB1 by inducing G0/G1 cell cycle arrest in ovarian cancer.
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引用次数: 0
Abstract
Background: Ovarian cancer is one of the leading causes of gynaecologic malignancies worldwide. This study aimed to explore the diagnostic and therapeutic potential of TMUB1 in ovarian cancer.
Methods: To investigate the subcellular localization of TMUB1 under serum deprivation conditions, immunofluorescence staining was performed. The cell cycle distribution was analysed by flow cytometry. TMUB1-interacting proteins were identified through immunoprecipitation followed by mass spectrometry. Kaplan-Meier survival analysis was used to compare overall survival between patients with high and low TMUB1 expression. Stromal and immune scores for tumour tissues in TCGA were calculated to assess the tumour microenvironment.
Results: TMUB1 plays a crucial role in regulating the cell cycle by promoting entry into the G0/G1 phase. Survival analysis revealed that TMUB1 expression is positively associated with favourable prognosis in patients with ovarian cancer. Pathway enrichment analysis highlighted several tumour-related pathways, including ECM-receptor interaction, JAK-STAT signalling, p53 signalling and apoptosis. The high TMUB1 expression group presented lower stromal scores. An inverse correlation was observed between TMUB1 expression and the expression of key immune checkpoint proteins.
Conclusions: TMUB1 might participate in the regulation of the G0/G1 cell cycle arrest, and it is a potential diagnostic marker and therapeutic target for ovarian cancer.
期刊介绍：
For a long time, solid neoplasms have been viewed as relatively homogeneous entities composed for the most part of malignant cells. It is now clear that tumors are highly heterogeneous structures that evolve in the context of intimate interactions between cancer cells and endothelial, stromal as well as immune cells. During the past few years, experimental and clinical oncologists have witnessed several conceptual transitions of this type. Molecular and Cellular Oncology (MCO) emerges within this conceptual framework as a high-profile forum for the publication of fundamental, translational and clinical research on cancer. The scope of MCO is broad. Submissions dealing with all aspects of oncogenesis, tumor progression and response to therapy will be welcome, irrespective of whether they focus on solid or hematological neoplasms. MCO has gathered leading scientists with expertise in multiple areas of cancer research and other fields of investigation to constitute a large, interdisciplinary, Editorial Board that will ensure the quality of articles accepted for publication. MCO will publish Original Research Articles, Brief Reports, Reviews, Short Reviews, Commentaries, Author Views (auto-commentaries) and Meeting Reports dealing with all aspects of cancer research.
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