Mixed circuit training as a non-pharmacological strategy to improve platelet function and oxidative balance in type 2 diabetes: role of purinergic signaling.

IF 2.5 4区医学 Q2 NEUROSCIENCES

Purinergic Signalling Pub Date : 2026-03-17 DOI:10.1007/s11302-026-10136-8

Lucas Macedo Chaves, Samantha Nuncio Prestes, André Campos De Lima, Aline Mânica, Daniela Zanini, Sedinei Lopes Copatti, Clodoaldo Antônio De Sá, Andréia Machado Cardoso

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Abstract

This study investigates the impact of a 16-week mixed circuit training (MCT) program on purinergic signaling and oxidative stress markers in women with type 2 diabetes mellitus (T2DM), focusing on its potential to modulate platelet-related purinergic signaling and oxidative stress markers that are mechanistically linked to platelet activation. A total of 21 women with T2DM and 23 non-diabetic controls, all sedentary and middle-aged, underwent MCT twice weekly. Biochemical, hemodynamic, and oxidative stress parameters, along with platelet ectonucleotidase activity and extracellular ATP levels, were assessed pre- and post-intervention. MCT significantly decreased ectonucleotidase diphosphohydrolase (E-NTPDase) activity for ADP hydrolysis in platelets, along with a reduction in extracellular ATP levels, indicating a modulation of purinergic signaling. Additionally, exercise enhanced antioxidant defenses, increasing glutathione-S-transferase (GST) activity and vitamin C levels, while reducing myeloperoxidase (MPO) activity, a key pro-oxidant enzyme. These changes suggest a shift toward a biochemical profile that may be relevant to thromboinflammatory pathways; however, no direct measures of platelet aggregation were performed. Mixed circuit training emerges as a valuable non-pharmacological strategy for improving oxidative balance and modulating purinergic markers in women with T2DM, warranting future studies including functional platelet assays and clinical outcomes.

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混合回路训练作为改善2型糖尿病血小板功能和氧化平衡的非药物策略：嘌呤能信号的作用

本研究探讨了16周混合回路训练（MCT）对2型糖尿病（T2DM）女性嘌呤能信号和氧化应激标志物的影响，重点关注其调节血小板相关嘌呤能信号和氧化应激标志物的潜力，这些标志物与血小板活化有机制联系。共有21名患有2型糖尿病的女性和23名非糖尿病对照者，都是久坐不动的中年人，每周接受两次MCT。评估干预前后的生化、血流动力学和氧化应激参数，以及血小板外核苷酸酶活性和细胞外ATP水平。MCT显著降低血小板中ADP水解的外核苷酸酶二磷酸水解酶（e - ntpase）活性，同时降低细胞外ATP水平，表明嘌呤能信号传导的调节。此外，运动增强抗氧化防御，增加谷胱甘肽- s -转移酶（GST）活性和维生素C水平，同时降低髓过氧化物酶（MPO）活性，这是一种关键的促氧化酶。这些变化提示可能与血栓炎症途径相关的生化特征的转变；然而，没有进行血小板聚集的直接测量。混合回路训练作为改善2型糖尿病女性氧化平衡和调节嘌呤能标志物的一种有价值的非药物策略，值得进一步研究，包括功能性血小板分析和临床结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Purinergic Signalling 医学-神经科学

CiteScore

6.60

自引率

17.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Nucleotides and nucleosides are primitive biological molecules that were utilized early in evolution both as intracellular energy sources and as extracellular signalling molecules. ATP was first identified as a neurotransmitter and later as a co-transmitter with all the established neurotransmitters in both peripheral and central nervous systems. Four subtypes of P1 (adenosine) receptors, 7 subtypes of P2X ion channel receptors and 8 subtypes of P2Y G protein-coupled receptors have currently been identified. Since P2 receptors were first cloned in the early 1990’s, there is clear evidence for the widespread distribution of both P1 and P2 receptor subtypes in neuronal and non-neuronal cells, including glial, immune, bone, muscle, endothelial, epithelial and endocrine cells.